Abstract
Airborne pathogenic microorganisms are hazardous bioaerosols which often cause serious respiratory diseases. To prevent airborne infectious disease, real-time detection and monitoring systems of airborne pathogens are needed. Since ATP (adenosine triphosphate) is a major biological energy source, the detection of ATP from aerosol reflects the existence of living microbes. Therefore, we developed a new biosensor to detect ATP from aerosols in real-time using an aerosol condensation system, a microfluidic channel, and an ATP-bioluminescence transducer. The condensation system enabled aerosol microbes (4 L) to be hydrosolized (0.2 ml) in 2 min. The bacterial intracellular ATP was then extracted in the passage through the microfluidic channel. The concentration of ATP could be determined by a bioluminescence sensor integrated in the channel. In this study, we used B. subtilis and E. coli JM110 as model airborne microbes. Our system can determine the existence of airborne microbes within 10 min. In the future, the application of our device will extend to the detection of fungi and consequently contribute to improving indoor air quality.
| Original language | English |
|---|---|
| Pages (from-to) | 443-448 |
| Number of pages | 6 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 132 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2008 Jun 16 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry
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A microfluidic ATP-bioluminescence sensor for the detection of airborne microbes. / Lee, Seung Jae; Park, Jae Sung; Im, Hee Taek; Jung, Hyo Il.
In: Sensors and Actuators, B: Chemical, Vol. 132, No. 2, 16.06.2008, p. 443-448.Research output: Contribution to journal › Article
TY - JOUR
T1 - A microfluidic ATP-bioluminescence sensor for the detection of airborne microbes
AU - Lee, Seung Jae
AU - Park, Jae Sung
AU - Im, Hee Taek
AU - Jung, Hyo Il
PY - 2008/6/16
Y1 - 2008/6/16
N2 - Airborne pathogenic microorganisms are hazardous bioaerosols which often cause serious respiratory diseases. To prevent airborne infectious disease, real-time detection and monitoring systems of airborne pathogens are needed. Since ATP (adenosine triphosphate) is a major biological energy source, the detection of ATP from aerosol reflects the existence of living microbes. Therefore, we developed a new biosensor to detect ATP from aerosols in real-time using an aerosol condensation system, a microfluidic channel, and an ATP-bioluminescence transducer. The condensation system enabled aerosol microbes (4 L) to be hydrosolized (0.2 ml) in 2 min. The bacterial intracellular ATP was then extracted in the passage through the microfluidic channel. The concentration of ATP could be determined by a bioluminescence sensor integrated in the channel. In this study, we used B. subtilis and E. coli JM110 as model airborne microbes. Our system can determine the existence of airborne microbes within 10 min. In the future, the application of our device will extend to the detection of fungi and consequently contribute to improving indoor air quality.
AB - Airborne pathogenic microorganisms are hazardous bioaerosols which often cause serious respiratory diseases. To prevent airborne infectious disease, real-time detection and monitoring systems of airborne pathogens are needed. Since ATP (adenosine triphosphate) is a major biological energy source, the detection of ATP from aerosol reflects the existence of living microbes. Therefore, we developed a new biosensor to detect ATP from aerosols in real-time using an aerosol condensation system, a microfluidic channel, and an ATP-bioluminescence transducer. The condensation system enabled aerosol microbes (4 L) to be hydrosolized (0.2 ml) in 2 min. The bacterial intracellular ATP was then extracted in the passage through the microfluidic channel. The concentration of ATP could be determined by a bioluminescence sensor integrated in the channel. In this study, we used B. subtilis and E. coli JM110 as model airborne microbes. Our system can determine the existence of airborne microbes within 10 min. In the future, the application of our device will extend to the detection of fungi and consequently contribute to improving indoor air quality.
UR - http://www.scopus.com/inward/record.url?scp=44649175643&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44649175643&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2007.10.035
DO - 10.1016/j.snb.2007.10.035
M3 - Article
AN - SCOPUS:44649175643
VL - 132
SP - 443
EP - 448
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 2
ER -