A microfluidic ATP-bioluminescence sensor for the detection of airborne microbes

Seung Jae Lee, Jae Sung Park, Hee Taek Im, Hyo Il Jung

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Airborne pathogenic microorganisms are hazardous bioaerosols which often cause serious respiratory diseases. To prevent airborne infectious disease, real-time detection and monitoring systems of airborne pathogens are needed. Since ATP (adenosine triphosphate) is a major biological energy source, the detection of ATP from aerosol reflects the existence of living microbes. Therefore, we developed a new biosensor to detect ATP from aerosols in real-time using an aerosol condensation system, a microfluidic channel, and an ATP-bioluminescence transducer. The condensation system enabled aerosol microbes (4 L) to be hydrosolized (0.2 ml) in 2 min. The bacterial intracellular ATP was then extracted in the passage through the microfluidic channel. The concentration of ATP could be determined by a bioluminescence sensor integrated in the channel. In this study, we used B. subtilis and E. coli JM110 as model airborne microbes. Our system can determine the existence of airborne microbes within 10 min. In the future, the application of our device will extend to the detection of fungi and consequently contribute to improving indoor air quality.

Original languageEnglish
Pages (from-to)443-448
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume132
Issue number2
DOIs
Publication statusPublished - 2008 Jun 16

Fingerprint

Bioluminescence
bioluminescence
adenosine triphosphate
microorganisms
Microfluidics
Aerosols
Adenosine Triphosphate
sensors
aerosols
Sensors
Condensation
Pulmonary diseases
Pathogens
respiratory diseases
condensation
Fungi
Air quality
Biosensors
Microorganisms
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Lee, Seung Jae ; Park, Jae Sung ; Im, Hee Taek ; Jung, Hyo Il. / A microfluidic ATP-bioluminescence sensor for the detection of airborne microbes. In: Sensors and Actuators, B: Chemical. 2008 ; Vol. 132, No. 2. pp. 443-448.
@article{3ce238761e3b48989a7da4057568b290,
title = "A microfluidic ATP-bioluminescence sensor for the detection of airborne microbes",
abstract = "Airborne pathogenic microorganisms are hazardous bioaerosols which often cause serious respiratory diseases. To prevent airborne infectious disease, real-time detection and monitoring systems of airborne pathogens are needed. Since ATP (adenosine triphosphate) is a major biological energy source, the detection of ATP from aerosol reflects the existence of living microbes. Therefore, we developed a new biosensor to detect ATP from aerosols in real-time using an aerosol condensation system, a microfluidic channel, and an ATP-bioluminescence transducer. The condensation system enabled aerosol microbes (4 L) to be hydrosolized (0.2 ml) in 2 min. The bacterial intracellular ATP was then extracted in the passage through the microfluidic channel. The concentration of ATP could be determined by a bioluminescence sensor integrated in the channel. In this study, we used B. subtilis and E. coli JM110 as model airborne microbes. Our system can determine the existence of airborne microbes within 10 min. In the future, the application of our device will extend to the detection of fungi and consequently contribute to improving indoor air quality.",
author = "Lee, {Seung Jae} and Park, {Jae Sung} and Im, {Hee Taek} and Jung, {Hyo Il}",
year = "2008",
month = "6",
day = "16",
doi = "10.1016/j.snb.2007.10.035",
language = "English",
volume = "132",
pages = "443--448",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "2",

}

A microfluidic ATP-bioluminescence sensor for the detection of airborne microbes. / Lee, Seung Jae; Park, Jae Sung; Im, Hee Taek; Jung, Hyo Il.

In: Sensors and Actuators, B: Chemical, Vol. 132, No. 2, 16.06.2008, p. 443-448.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A microfluidic ATP-bioluminescence sensor for the detection of airborne microbes

AU - Lee, Seung Jae

AU - Park, Jae Sung

AU - Im, Hee Taek

AU - Jung, Hyo Il

PY - 2008/6/16

Y1 - 2008/6/16

N2 - Airborne pathogenic microorganisms are hazardous bioaerosols which often cause serious respiratory diseases. To prevent airborne infectious disease, real-time detection and monitoring systems of airborne pathogens are needed. Since ATP (adenosine triphosphate) is a major biological energy source, the detection of ATP from aerosol reflects the existence of living microbes. Therefore, we developed a new biosensor to detect ATP from aerosols in real-time using an aerosol condensation system, a microfluidic channel, and an ATP-bioluminescence transducer. The condensation system enabled aerosol microbes (4 L) to be hydrosolized (0.2 ml) in 2 min. The bacterial intracellular ATP was then extracted in the passage through the microfluidic channel. The concentration of ATP could be determined by a bioluminescence sensor integrated in the channel. In this study, we used B. subtilis and E. coli JM110 as model airborne microbes. Our system can determine the existence of airborne microbes within 10 min. In the future, the application of our device will extend to the detection of fungi and consequently contribute to improving indoor air quality.

AB - Airborne pathogenic microorganisms are hazardous bioaerosols which often cause serious respiratory diseases. To prevent airborne infectious disease, real-time detection and monitoring systems of airborne pathogens are needed. Since ATP (adenosine triphosphate) is a major biological energy source, the detection of ATP from aerosol reflects the existence of living microbes. Therefore, we developed a new biosensor to detect ATP from aerosols in real-time using an aerosol condensation system, a microfluidic channel, and an ATP-bioluminescence transducer. The condensation system enabled aerosol microbes (4 L) to be hydrosolized (0.2 ml) in 2 min. The bacterial intracellular ATP was then extracted in the passage through the microfluidic channel. The concentration of ATP could be determined by a bioluminescence sensor integrated in the channel. In this study, we used B. subtilis and E. coli JM110 as model airborne microbes. Our system can determine the existence of airborne microbes within 10 min. In the future, the application of our device will extend to the detection of fungi and consequently contribute to improving indoor air quality.

UR - http://www.scopus.com/inward/record.url?scp=44649175643&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44649175643&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2007.10.035

DO - 10.1016/j.snb.2007.10.035

M3 - Article

AN - SCOPUS:44649175643

VL - 132

SP - 443

EP - 448

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

IS - 2

ER -