Abstract
This paper describes a custom-designed microfluidic chip for sensitive detection of antibody using quantum dot fluorescence in a microbead-based assay. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies happens on the surface. The microfluidic chip is fabricated on a glass substrate using transparent silicone elastomer, PDMS, for easy access to monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assay has been performed in the fabricated chip including: capturing of microbeads; antibody injection into a micro-chamber; quantum dot injection; and fluorescence detection. Various concentrations of antibody have been tested and have shown good agreement with correlated fluorescent intensities.
| Original language | English |
|---|---|
| Article number | S10 |
| Pages (from-to) | 3178-3183 |
| Number of pages | 6 |
| Journal | Measurement Science and Technology |
| Volume | 17 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2006 Dec 1 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Instrumentation
- Engineering (miscellaneous)
- Applied Mathematics
Cite this
}
A microfluidic chip for measurement of biomolecules using a microbead-based quantum dot fluorescence assay. / Yun, Kwang Seok; Lee, Dohoon; Kim, Hak Sung; Yoon, Euisik.
In: Measurement Science and Technology, Vol. 17, No. 12, S10, 01.12.2006, p. 3178-3183.Research output: Contribution to journal › Article
TY - JOUR
T1 - A microfluidic chip for measurement of biomolecules using a microbead-based quantum dot fluorescence assay
AU - Yun, Kwang Seok
AU - Lee, Dohoon
AU - Kim, Hak Sung
AU - Yoon, Euisik
PY - 2006/12/1
Y1 - 2006/12/1
N2 - This paper describes a custom-designed microfluidic chip for sensitive detection of antibody using quantum dot fluorescence in a microbead-based assay. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies happens on the surface. The microfluidic chip is fabricated on a glass substrate using transparent silicone elastomer, PDMS, for easy access to monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assay has been performed in the fabricated chip including: capturing of microbeads; antibody injection into a micro-chamber; quantum dot injection; and fluorescence detection. Various concentrations of antibody have been tested and have shown good agreement with correlated fluorescent intensities.
AB - This paper describes a custom-designed microfluidic chip for sensitive detection of antibody using quantum dot fluorescence in a microbead-based assay. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies happens on the surface. The microfluidic chip is fabricated on a glass substrate using transparent silicone elastomer, PDMS, for easy access to monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assay has been performed in the fabricated chip including: capturing of microbeads; antibody injection into a micro-chamber; quantum dot injection; and fluorescence detection. Various concentrations of antibody have been tested and have shown good agreement with correlated fluorescent intensities.
UR - http://www.scopus.com/inward/record.url?scp=33846040371&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846040371&partnerID=8YFLogxK
U2 - 10.1088/0957-0233/17/12/S10
DO - 10.1088/0957-0233/17/12/S10
M3 - Article
AN - SCOPUS:33846040371
VL - 17
SP - 3178
EP - 3183
JO - Measurement Science and Technology
JF - Measurement Science and Technology
SN - 0957-0233
IS - 12
M1 - S10
ER -