A microfluidic chip for measurement of biomolecules using a microbead-based quantum dot fluorescence assay

Kwang Seok Yun, Dohoon Lee, Hak Sung Kim, Euisik Yoon

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This paper describes a custom-designed microfluidic chip for sensitive detection of antibody using quantum dot fluorescence in a microbead-based assay. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies happens on the surface. The microfluidic chip is fabricated on a glass substrate using transparent silicone elastomer, PDMS, for easy access to monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assay has been performed in the fabricated chip including: capturing of microbeads; antibody injection into a micro-chamber; quantum dot injection; and fluorescence detection. Various concentrations of antibody have been tested and have shown good agreement with correlated fluorescent intensities.

Original languageEnglish
Article numberS10
Pages (from-to)3178-3183
Number of pages6
JournalMeasurement Science and Technology
Volume17
Issue number12
DOIs
Publication statusPublished - 2006 Dec 1

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Engineering (miscellaneous)
  • Applied Mathematics

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