A microfluidic chip for measurement of biomolecules using a microbead-based quantum dot fluorescence assay

Kwang Seok Yun, Dohoon Lee, Hak Sung Kim, Euisik Yoon

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This paper describes a custom-designed microfluidic chip for sensitive detection of antibody using quantum dot fluorescence in a microbead-based assay. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies happens on the surface. The microfluidic chip is fabricated on a glass substrate using transparent silicone elastomer, PDMS, for easy access to monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assay has been performed in the fabricated chip including: capturing of microbeads; antibody injection into a micro-chamber; quantum dot injection; and fluorescence detection. Various concentrations of antibody have been tested and have shown good agreement with correlated fluorescent intensities.

Original languageEnglish
Article numberS10
Pages (from-to)3178-3183
Number of pages6
JournalMeasurement Science and Technology
Volume17
Issue number12
DOIs
Publication statusPublished - 2006 Dec 1

Fingerprint

Biomolecules
Microfluidics
Quantum Dots
antibodies
Antibody
Antibodies
Fluorescence
Semiconductor quantum dots
Assays
Chip
chips
quantum dots
fluorescence
Injection
injection
Elastomers
silicones
elastomers
Silicones
chambers

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Engineering (miscellaneous)
  • Applied Mathematics

Cite this

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A microfluidic chip for measurement of biomolecules using a microbead-based quantum dot fluorescence assay. / Yun, Kwang Seok; Lee, Dohoon; Kim, Hak Sung; Yoon, Euisik.

In: Measurement Science and Technology, Vol. 17, No. 12, S10, 01.12.2006, p. 3178-3183.

Research output: Contribution to journalArticle

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