TY - JOUR
T1 - A microfluidic chip for measurement of biomolecules using a microbead-based quantum dot fluorescence assay
AU - Yun, Kwang Seok
AU - Lee, Dohoon
AU - Kim, Hak Sung
AU - Yoon, Euisik
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2006/12/1
Y1 - 2006/12/1
N2 - This paper describes a custom-designed microfluidic chip for sensitive detection of antibody using quantum dot fluorescence in a microbead-based assay. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies happens on the surface. The microfluidic chip is fabricated on a glass substrate using transparent silicone elastomer, PDMS, for easy access to monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assay has been performed in the fabricated chip including: capturing of microbeads; antibody injection into a micro-chamber; quantum dot injection; and fluorescence detection. Various concentrations of antibody have been tested and have shown good agreement with correlated fluorescent intensities.
AB - This paper describes a custom-designed microfluidic chip for sensitive detection of antibody using quantum dot fluorescence in a microbead-based assay. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies happens on the surface. The microfluidic chip is fabricated on a glass substrate using transparent silicone elastomer, PDMS, for easy access to monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assay has been performed in the fabricated chip including: capturing of microbeads; antibody injection into a micro-chamber; quantum dot injection; and fluorescence detection. Various concentrations of antibody have been tested and have shown good agreement with correlated fluorescent intensities.
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U2 - 10.1088/0957-0233/17/12/S10
DO - 10.1088/0957-0233/17/12/S10
M3 - Article
AN - SCOPUS:33846040371
VL - 17
SP - 3178
EP - 3183
JO - Measurement Science and Technology
JF - Measurement Science and Technology
SN - 0957-0233
IS - 12
M1 - S10
ER -