A microfluidic electrochemical aptasensor for enrichment and detection of bisphenol A.

Leila Kashefi-Kheyrabadi, Junmoo Kim, Hogyeong Gwak, Kyung A. Hyun, Nam Ho Bae, Seok Jae Lee, Hyo il Jung

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Bisphenol A (BPA) is an organic monomer used to make common consumer goods such as plastic containers, sports equipment, and cosmetics which are heavily produced worldwide. A growing interest has been drawn to general public as BPA is one of the major endocrine disrupting chemicals threating human health. To date, numerous BPA sensors have been attempted to be developed but important challenges still remained such as limited linearity range, easy to use, and long term response time. To address the present issues, a microfluidic channel should be integrated into an electrochemical aptasensor and it is called Geometrically Activated Surface Interaction (GASI) chip. The vigorous generation of the micro-vortex in the GASI fluidic chamber provides the high collision chances between BPA and anti-BPA aptamer (BPAPT) and consequently more BPA molecules can be captured on the aptasensor surface, which finally results in high sensitivity of the aptasensor. To construct the integrated aptasensor, a miniaturized gold electrode is fabricated using shadow mask and e-beam evaporation process. Afterward, BPAPT is immobilized on a nanostructured gold electrode via thiol chemistry, and other terminus of the aptamer is labeled with a ferrocene (Fc) redox probe. Then, the microfluidic channel is mounted over the miniaturized gold electrode to introduce and enrich BPA to the aptasensor. Upon the specific interaction between BPA and its aptamer, configuration of aptamer is changed so that Fc tag approaches to the electrode surface and direct oxidation signal of Fc and BPA are followed as analytical signals. The unique microfluidic integrated electrochemical aptasensor delivers a wide linear dynamic range over 5 × 10–12 to 1 × 10−9 M, with a limit of detection 2 × 10–13 M. This aptasensor provides a precise platform for simple, selective and more importantly rapid detection of BPA. Such kind of sensing platforms can serve as a fertile ground for designing miniaturized portable sensors.

Original languageEnglish
Pages (from-to)457-463
Number of pages7
JournalBiosensors and Bioelectronics
Volume117
DOIs
Publication statusPublished - 2018 Oct 15

Fingerprint

Microfluidics
Electrodes
Gold
Plastic containers
Cosmetics
Sensors
Fluidics
Sports
Masks
Microelectrodes
Evaporation
Vortex flow
Monomers
Health
Sports Equipment
Oxidation
Molecules
bisphenol A
Endocrine Disruptors
Social Justice

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Kashefi-Kheyrabadi, Leila ; Kim, Junmoo ; Gwak, Hogyeong ; Hyun, Kyung A. ; Bae, Nam Ho ; Lee, Seok Jae ; Jung, Hyo il. / A microfluidic electrochemical aptasensor for enrichment and detection of bisphenol A. In: Biosensors and Bioelectronics. 2018 ; Vol. 117. pp. 457-463.
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abstract = "Bisphenol A (BPA) is an organic monomer used to make common consumer goods such as plastic containers, sports equipment, and cosmetics which are heavily produced worldwide. A growing interest has been drawn to general public as BPA is one of the major endocrine disrupting chemicals threating human health. To date, numerous BPA sensors have been attempted to be developed but important challenges still remained such as limited linearity range, easy to use, and long term response time. To address the present issues, a microfluidic channel should be integrated into an electrochemical aptasensor and it is called Geometrically Activated Surface Interaction (GASI) chip. The vigorous generation of the micro-vortex in the GASI fluidic chamber provides the high collision chances between BPA and anti-BPA aptamer (BPAPT) and consequently more BPA molecules can be captured on the aptasensor surface, which finally results in high sensitivity of the aptasensor. To construct the integrated aptasensor, a miniaturized gold electrode is fabricated using shadow mask and e-beam evaporation process. Afterward, BPAPT is immobilized on a nanostructured gold electrode via thiol chemistry, and other terminus of the aptamer is labeled with a ferrocene (Fc) redox probe. Then, the microfluidic channel is mounted over the miniaturized gold electrode to introduce and enrich BPA to the aptasensor. Upon the specific interaction between BPA and its aptamer, configuration of aptamer is changed so that Fc tag approaches to the electrode surface and direct oxidation signal of Fc and BPA are followed as analytical signals. The unique microfluidic integrated electrochemical aptasensor delivers a wide linear dynamic range over 5 × 10–12 to 1 × 10−9 M, with a limit of detection 2 × 10–13 M. This aptasensor provides a precise platform for simple, selective and more importantly rapid detection of BPA. Such kind of sensing platforms can serve as a fertile ground for designing miniaturized portable sensors.",
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A microfluidic electrochemical aptasensor for enrichment and detection of bisphenol A. / Kashefi-Kheyrabadi, Leila; Kim, Junmoo; Gwak, Hogyeong; Hyun, Kyung A.; Bae, Nam Ho; Lee, Seok Jae; Jung, Hyo il.

In: Biosensors and Bioelectronics, Vol. 117, 15.10.2018, p. 457-463.

Research output: Contribution to journalArticle

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