A microfluidic magnetic bead impact generator for physical stimulation of osteoblast cell

Suk Heung Song, Jeongheon Choi, Hyo Il Jung

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

We developed a novel microfluidic cell culture device in which magnetic beads repetitively collide with osteoblast cells, MC3T3-E1, owing to attractive forces generated by pulsed electromagnetic fields and consequently the cells were physically stimulated by bead impacts. Our device consists of an on-chip microelectromagnet and a microfluidic channel which were fabricated by a microelectromechanical system technique. The impact forces and stresses acting on a cell were numerically analyzed and experimentally generated with different sizes of bead (4.5, 7.6 and 8.4 μm) and at various pulse frequencies (60 Hz, 1 kHz and 1 MHz). Cells were synchronized at each specific phase of the cell cycle before stimulation in order to determine the most susceptible phase against bead impacts. The cells were stimulated with different sizes of bead at various pulse frequencies for 1 min at G1, S and G2 phases, respectively, and then counted immediately after one doubling time. The growth rate of cells was highly accelerated when they were stimulated with 4.5 μm beads at G1 phase and a pulse frequency of 1 MHz. Almost all of the cells were viable after stimulation, indicating that our cell stimulator did not cause any cellular damage and is suitable for use in new physical stimulus modalities.

Original languageEnglish
Pages (from-to)2762-2770
Number of pages9
JournalElectrophoresis
Volume31
Issue number16
DOIs
Publication statusPublished - 2010 Aug

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'A microfluidic magnetic bead impact generator for physical stimulation of osteoblast cell'. Together they form a unique fingerprint.

Cite this