TY - GEN
T1 - A micromixer for continuous labeling of circulating tumor cells with micro-beads as a highly selective isolation
AU - Lin, Ming Xian
AU - Hyun, Kyung A.
AU - Moon, Hui Sung
AU - Sim, Tae Seok
AU - Lee, Jeong Gun
AU - Park, Jae Chan
AU - Jung, Hyo Il
PY - 2012
Y1 - 2012
N2 - Rare circulating tumor cells (CTCs) have been identified in peripheral blood from cancer patients and have been proved to be a main cause of metastatic disease. Current strategies for size based on isolation of CTCs has been technically challenged owing to their overlapped size value(e.g., CTCs ~16-20μm diameter and leukocytes ~8-14 μm). Here we describe the development of a unique microfluidic device, Taylor Gortler(TG) mixer, capable of efficient and selective separation of viable CTCs from peripheral whole blood samples, operated by the interaction of target CTCs with antibody (EpCAM)-coated microbeads under microfluidic channel, as a result the size of the CTCs will be increased. First of all, we used red-green fluorescence beads to evaluate the mixing efficiency of the device. And then, we have gotten binding efficiency by using breast cancer cell and anti-epithelial cell adhesion molecule(EpCAM) coated microbeads through TG mixer.
AB - Rare circulating tumor cells (CTCs) have been identified in peripheral blood from cancer patients and have been proved to be a main cause of metastatic disease. Current strategies for size based on isolation of CTCs has been technically challenged owing to their overlapped size value(e.g., CTCs ~16-20μm diameter and leukocytes ~8-14 μm). Here we describe the development of a unique microfluidic device, Taylor Gortler(TG) mixer, capable of efficient and selective separation of viable CTCs from peripheral whole blood samples, operated by the interaction of target CTCs with antibody (EpCAM)-coated microbeads under microfluidic channel, as a result the size of the CTCs will be increased. First of all, we used red-green fluorescence beads to evaluate the mixing efficiency of the device. And then, we have gotten binding efficiency by using breast cancer cell and anti-epithelial cell adhesion molecule(EpCAM) coated microbeads through TG mixer.
UR - http://www.scopus.com/inward/record.url?scp=84901780838&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901780838&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901780838
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1681
EP - 1683
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -
