A micromixer for continuous labeling of circulating tumor cells with micro-beads as a highly selective isolation

Ming Xian Lin, Kyung A. Hyun, Hui Sung Moon, Tae Seok Sim, Jeong Gun Lee, Jae Chan Park, Hyo Il Jung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Rare circulating tumor cells (CTCs) have been identified in peripheral blood from cancer patients and have been proved to be a main cause of metastatic disease. Current strategies for size based on isolation of CTCs has been technically challenged owing to their overlapped size value(e.g., CTCs ~16-20μm diameter and leukocytes ~8-14 μm). Here we describe the development of a unique microfluidic device, Taylor Gortler(TG) mixer, capable of efficient and selective separation of viable CTCs from peripheral whole blood samples, operated by the interaction of target CTCs with antibody (EpCAM)-coated microbeads under microfluidic channel, as a result the size of the CTCs will be increased. First of all, we used red-green fluorescence beads to evaluate the mixing efficiency of the device. And then, we have gotten binding efficiency by using breast cancer cell and anti-epithelial cell adhesion molecule(EpCAM) coated microbeads through TG mixer.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1681-1683
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Fingerprint

Labeling
Tumors
Cells
Cell adhesion
Cell Adhesion Molecules
Microfluidics
Blood
Molecules
Antibodies
Fluorescence
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Lin, M. X., Hyun, K. A., Moon, H. S., Sim, T. S., Lee, J. G., Park, J. C., & Jung, H. I. (2012). A micromixer for continuous labeling of circulating tumor cells with micro-beads as a highly selective isolation. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1681-1683). Chemical and Biological Microsystems Society.
Lin, Ming Xian ; Hyun, Kyung A. ; Moon, Hui Sung ; Sim, Tae Seok ; Lee, Jeong Gun ; Park, Jae Chan ; Jung, Hyo Il. / A micromixer for continuous labeling of circulating tumor cells with micro-beads as a highly selective isolation. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 1681-1683
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Lin, MX, Hyun, KA, Moon, HS, Sim, TS, Lee, JG, Park, JC & Jung, HI 2012, A micromixer for continuous labeling of circulating tumor cells with micro-beads as a highly selective isolation. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 1681-1683, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 12/10/28.

A micromixer for continuous labeling of circulating tumor cells with micro-beads as a highly selective isolation. / Lin, Ming Xian; Hyun, Kyung A.; Moon, Hui Sung; Sim, Tae Seok; Lee, Jeong Gun; Park, Jae Chan; Jung, Hyo Il.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1681-1683.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Lin MX, Hyun KA, Moon HS, Sim TS, Lee JG, Park JC et al. A micromixer for continuous labeling of circulating tumor cells with micro-beads as a highly selective isolation. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1681-1683