A miRNA-Mediated Approach to Dissect the Complexity of Tumor-Initiating Cell Function and Identify miRNA-Targeting Drugs

Anil Belur Nagaraj, Peronne Joseph, Erin Ponting, Yuriy Fedorov, Salendra Singh, Alex Cole, Woncheol Lee, Euisik Yoon, Alessia Baccarini, Peter Scacheri, Ronald Buckanovich, Drew J. Adams, Ronny Drapkin, Brian D. Brown, Analisa DiFeo

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

In this article, DiFeo and colleagues have utilized a miRNA 3′ UTR biosensor to study tumor-initiating cells in ovarian cancer and develop a high-throughput platform to uncover miRNA-targeting drugs. They have identified miR-181a as a potent driver of tumor initiation, which can be regulated by BET inhibitors, thus introducing a previously unknown biomarker for these drugs.

Original languageEnglish
Pages (from-to)122-134
Number of pages13
JournalStem Cell Reports
Volume12
Issue number1
DOIs
Publication statusPublished - 2019 Jan 8

Bibliographical note

Funding Information:
We thank Norma C. and Albert I. Geller for their constant support of the Gynecological Cancer Translational Research Program at Case Western Reserve University (A.D.). In addition, we thank Dr. Anirban Mitra for the HEYA8 cells, Dr. Goutham Narla for the MDA-MB-231 and H358 cells, and Dr. Steven Presnell for the miR-181a luciferase promoter. We acknowledge the help from Cytometry & Imaging Microscopy Core Facility and the Athymic Animal and Preclinical Therapeutics Core of the Case Comprehensive Cancer Center ( P30CA043703 ). This work was supported by grants from The National Cancer Institute , R01CA197780 (A.D.), Department of Defense , OC150553 (A.D.), and The Young Scientist Foundation (A.D.).

Funding Information:
We thank Norma C. and Albert I. Geller for their constant support of the Gynecological Cancer Translational Research Program at Case Western Reserve University (A.D.). In addition, we thank Dr. Anirban Mitra for the HEYA8 cells, Dr. Goutham Narla for the MDA-MB-231 and H358 cells, and Dr. Steven Presnell for the miR-181a luciferase promoter. We acknowledge the help from Cytometry & Imaging Microscopy Core Facility and the Athymic Animal and Preclinical Therapeutics Core of the Case Comprehensive Cancer Center (P30CA043703). This work was supported by grants from The National Cancer Institute, R01CA197780 (A.D.), Department of Defense, OC150553 (A.D.), and The Young Scientist Foundation (A.D.).

Publisher Copyright:
© 2018 The Authors

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

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