A modular microfluidic platform for serial enrichment and harvest of pure extracellular vesicles†

Hogyeong Gwak, Sunyoung Park, Haeun Yu, Kyung A. Hyun, Hyo Il Jung

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Extracellular vesicles (EVs) are recognized as promising biomarkers for several diseases. However, their conventional isolation methods have several drawbacks, such as poor yields, low purity, and time-consuming operations. Therefore, a simple, low-cost, and rapid microfluidic platform has been extensively developed to meet the requirement in biomedical applications. Herein, a modular microfluidic platform is demonstrated to isolate and enrich EVs directly from plasma, in a combination of continuous capture and purification of EVs. The EVs were selectively captured by target-specific antibody-coated beads in a horseshoe-shaped orifice micromixer (HOMM) chip within 2 min. A fish-trap-shaped microfilter unit was subsequently used to elute and purify the affinity-induced captured EVs from the microbeads. The ability of the modular chip to capture, enrich, and release EVs was demonstrated in 5 min (100 μL sample) at high throughput (100 μL min−1). The two chips can be modularized or individually operated, depending on the clinical applications such as diagnostics and therapeutics. For the diagnostic applications, the EVs on microbeads can be directly subjected to the molecular analysis whereas the pure EVs should be released from the microbeads for the therapeutic treatments. This study reveals that the fabricated modular chip can be appropriately employed as a platform for EV-related research tools.

Original languageEnglish
Pages (from-to)1117-1127
Number of pages11
Issue number6
Publication statusPublished - 2022 Feb 22

Bibliographical note

Funding Information:
This work was supported by the Technology Innovation Program (20008829) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea) (20008829), 2021Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2021R1C1C2007646), and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2020M3A9I4039045 and No. 2021R1A2C301125411).

Publisher Copyright:
This journal is © The Royal Society of Chemistry

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry


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