Abstract
Paraneoplastic pemphigus (PNP) is an acquired autoimmune disorder characterized by severe mucosal erosion, and polymorphous cutaneous lesions associated with neoplasia. PNP patients have circulating autoantibodies that bind to stratified and nonstratified epithelia. Previously, we showed that envoplakin was a component of the antigen complex recognized by PNP sera. In the present study we generated a monoclonal antibody, EVP-YS, against human envoplakin. The antibody bound to keratinocyte cell surfaces and reacted with the 210-kDa PNP antigen, confirming its specificity for envoplakin. The variable regions of the heavy (H) and light (L) chain genes were cloned from the hybridoma and shown to belong to mouse H chain subgroup III and κ light chain subgroup V, respectively. The L chain of EVP-YS was 98% identical to the κ chains of some autoantibodies and anti-nucleic acid antibodies, and had an identical amino acid sequence in all three complementary determining regions, suggesting that the H chains determine the specificity of the EVP-YS-envoplakin interaction. The EVP-YS antibody can be used to evaluate the sensitivity and specificity of clinical, histological, and immunological criteria for diagnosing PNP.
| Original language | English |
|---|---|
| Pages (from-to) | 237-241 |
| Number of pages | 5 |
| Journal | Molecules and cells |
| Volume | 18 |
| Issue number | 2 |
| Publication status | Published - 2004 Oct 31 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology
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A monoclonal antibody against the paraneoplastic pemphigus (PNP) antigen, envoplakin : cDNA sequences encoding the variable regions of heavy and light chains. / Ahn, Jin Hyung; Lee, Min Geol; Kim, Soo Chan; Lee, Tae Ho.
In: Molecules and cells, Vol. 18, No. 2, 31.10.2004, p. 237-241.Research output: Contribution to journal › Article
TY - JOUR
T1 - A monoclonal antibody against the paraneoplastic pemphigus (PNP) antigen, envoplakin
T2 - cDNA sequences encoding the variable regions of heavy and light chains
AU - Ahn, Jin Hyung
AU - Lee, Min Geol
AU - Kim, Soo Chan
AU - Lee, Tae Ho
PY - 2004/10/31
Y1 - 2004/10/31
N2 - Paraneoplastic pemphigus (PNP) is an acquired autoimmune disorder characterized by severe mucosal erosion, and polymorphous cutaneous lesions associated with neoplasia. PNP patients have circulating autoantibodies that bind to stratified and nonstratified epithelia. Previously, we showed that envoplakin was a component of the antigen complex recognized by PNP sera. In the present study we generated a monoclonal antibody, EVP-YS, against human envoplakin. The antibody bound to keratinocyte cell surfaces and reacted with the 210-kDa PNP antigen, confirming its specificity for envoplakin. The variable regions of the heavy (H) and light (L) chain genes were cloned from the hybridoma and shown to belong to mouse H chain subgroup III and κ light chain subgroup V, respectively. The L chain of EVP-YS was 98% identical to the κ chains of some autoantibodies and anti-nucleic acid antibodies, and had an identical amino acid sequence in all three complementary determining regions, suggesting that the H chains determine the specificity of the EVP-YS-envoplakin interaction. The EVP-YS antibody can be used to evaluate the sensitivity and specificity of clinical, histological, and immunological criteria for diagnosing PNP.
AB - Paraneoplastic pemphigus (PNP) is an acquired autoimmune disorder characterized by severe mucosal erosion, and polymorphous cutaneous lesions associated with neoplasia. PNP patients have circulating autoantibodies that bind to stratified and nonstratified epithelia. Previously, we showed that envoplakin was a component of the antigen complex recognized by PNP sera. In the present study we generated a monoclonal antibody, EVP-YS, against human envoplakin. The antibody bound to keratinocyte cell surfaces and reacted with the 210-kDa PNP antigen, confirming its specificity for envoplakin. The variable regions of the heavy (H) and light (L) chain genes were cloned from the hybridoma and shown to belong to mouse H chain subgroup III and κ light chain subgroup V, respectively. The L chain of EVP-YS was 98% identical to the κ chains of some autoantibodies and anti-nucleic acid antibodies, and had an identical amino acid sequence in all three complementary determining regions, suggesting that the H chains determine the specificity of the EVP-YS-envoplakin interaction. The EVP-YS antibody can be used to evaluate the sensitivity and specificity of clinical, histological, and immunological criteria for diagnosing PNP.
UR - http://www.scopus.com/inward/record.url?scp=11444268287&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=11444268287&partnerID=8YFLogxK
M3 - Article
C2 - 15529001
AN - SCOPUS:11444268287
VL - 18
SP - 237
EP - 241
JO - Molecules and Cells
JF - Molecules and Cells
SN - 1016-8478
IS - 2
ER -