A multi-layered microfluidic device formagnetophoretic cell separation

Hye Lyn Lee, Suk Heung Song, Hee Taek Lim, Hyung Joon Kim, Min Suk Park, Hyo Il Jung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we present the design and experimental results of a multi-layered microfluidic electromagnetic cell separation device. Our channel consists of top and bottom layers in order to separate magnetically labeled cells in the vertical direction. Rapid separation of magnetic beads in top and bottom channel can be used in high throughput screening to monitor the efficacy and drug compounds. The experiments using the device were carried out with 4.5|j.m magnetic bead and magnetic labeled Jurkat cell under electromagnetic field of 1.55mT. Without the magnetic field, the magnetic labeled cells started to flow from the bottom inlet and exit out of the bottom channel outlet. In the presence of the magnetic field, the cells started in bottom channel are attracted upward by the electromagnetic field and flow through the top layered. Finally, the labeled cells flow out the top channel outlet. The separation efficiencies of the multi-layer structured microfluidic channel showed more than 95%. We found that the multi-layer structured microfluidic channel was very effective in enhancing the separation. This microfluidic channel can be potentially applied to Lab-on-a-chip system because of its attractive features such as high throughput, continuous sorting, simple and rapid fabrication.

Original languageEnglish
Title of host publicationBIODEVICES 2009 - Proceedings of the 2nd International Conference on Biomedical Electronics and Devices
Pages286-289
Number of pages4
Publication statusPublished - 2009
Event2nd International Conference on Biomedical Electronics and Devices, BIODEVICES 2009 - Porto, Portugal
Duration: 2009 Jan 142009 Jan 17

Publication series

NameBIODEVICES 2009 - Proceedings of the 2nd International Conference on Biomedical Electronics and Devices

Other

Other2nd International Conference on Biomedical Electronics and Devices, BIODEVICES 2009
CountryPortugal
CityPorto
Period09/1/1409/1/17

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Lee, H. L., Song, S. H., Lim, H. T., Kim, H. J., Park, M. S., & Jung, H. I. (2009). A multi-layered microfluidic device formagnetophoretic cell separation. In BIODEVICES 2009 - Proceedings of the 2nd International Conference on Biomedical Electronics and Devices (pp. 286-289). (BIODEVICES 2009 - Proceedings of the 2nd International Conference on Biomedical Electronics and Devices).