A multimodal holographic system for optical manipulation and injection of developing embryos

Maria Leilani Torres-Mapa, MacIej Antkowiak, Hana Cizmarova, David E.K. Ferrier, Kishan Dholakia, Frank Gunn-Moore

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Transgenic animals are an essential means for investigating genetic processes in vivo, and depend on efficient delivery techniques to introduce exogenous genetic material into the organism, often at the zygote stage. In this study, we demonstrate an optical approach to microinjection based on a holographic system using a spatial light modulator and a Ti: Sapphire laser. This integrated system is capable of both optical orientation and injection of 60- μm diameter Pomatoceros lamarckii (P.lamarckii) embryos. Individual blastomeres of P. lamarckii embryos were optoinjected with varying sizes of dextran molecules and Propidium iodide using an 800-nm femtosecond laser with controlled dosage. We also show that the technique is able to deliver materials to cells located deep within a welldeveloped embryo. As a visual confirmation of successful optoinjection, the presence of gas bubbles was observed as a function of laser power and exposure time. Small gas bubbles, less than 5-μm in diameter, were found to be tolerated by the irradiated embryo. Furthermore, when switched to the continuous wave mode, the laser could exert optical forces upon the embryo. This facilitated computer-controlled handling and orientation of P. lamarckii embryos without compromising viability. Our multimodal optical platform offers a sterile, non-contact and robust alternative to traditional microinjection. This work is a step towards applications in developmental biology such as cell lineage mapping and formation of transgenic animals using an optical approach.

Original languageEnglish
Title of host publicationBiophotonics
Subtitle of host publicationPhotonic Solutions for Better Health Care III
DOIs
Publication statusPublished - 2012 Jun 12
EventBiophotonics: Photonic Solutions for Better Health Care III - Brussels, Belgium
Duration: 2012 Apr 162012 Apr 19

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8427
ISSN (Print)1605-7422

Conference

ConferenceBiophotonics: Photonic Solutions for Better Health Care III
CountryBelgium
CityBrussels
Period12/4/1612/4/19

Fingerprint

Optical Devices
embryos
manipulators
Embryonic Structures
injection
Injections
Lasers
Animals
Gases
Genetically Modified Animals
Dextran
Propidium
Aluminum Oxide
Microinjections
Laser modes
Dextrans
Ultrashort pulses
Sapphire
lasers
animals

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Torres-Mapa, M. L., Antkowiak, M., Cizmarova, H., Ferrier, D. E. K., Dholakia, K., & Gunn-Moore, F. (2012). A multimodal holographic system for optical manipulation and injection of developing embryos. In Biophotonics: Photonic Solutions for Better Health Care III [84273D] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8427). https://doi.org/10.1117/12.922509
Torres-Mapa, Maria Leilani ; Antkowiak, MacIej ; Cizmarova, Hana ; Ferrier, David E.K. ; Dholakia, Kishan ; Gunn-Moore, Frank. / A multimodal holographic system for optical manipulation and injection of developing embryos. Biophotonics: Photonic Solutions for Better Health Care III. 2012. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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Torres-Mapa, ML, Antkowiak, M, Cizmarova, H, Ferrier, DEK, Dholakia, K & Gunn-Moore, F 2012, A multimodal holographic system for optical manipulation and injection of developing embryos. in Biophotonics: Photonic Solutions for Better Health Care III., 84273D, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8427, Biophotonics: Photonic Solutions for Better Health Care III, Brussels, Belgium, 12/4/16. https://doi.org/10.1117/12.922509

A multimodal holographic system for optical manipulation and injection of developing embryos. / Torres-Mapa, Maria Leilani; Antkowiak, MacIej; Cizmarova, Hana; Ferrier, David E.K.; Dholakia, Kishan; Gunn-Moore, Frank.

Biophotonics: Photonic Solutions for Better Health Care III. 2012. 84273D (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8427).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Torres-Mapa ML, Antkowiak M, Cizmarova H, Ferrier DEK, Dholakia K, Gunn-Moore F. A multimodal holographic system for optical manipulation and injection of developing embryos. In Biophotonics: Photonic Solutions for Better Health Care III. 2012. 84273D. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.922509