A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II

Young Joon Kim, Stefan Björklund, Yang Li, Michael H. Sayre, Roger D. Kornberg

Research output: Contribution to journalArticle

820 Citations (Scopus)

Abstract

A mediator was isolated from yeast that enabled a response to the activator proteins GAL4-VP16 and GCN4 in a transcription system reconstituted with essentially homogeneous basal factors and RNA polymerase II. The mediator comprised some 20 polypeptides, including the three subunits of TFIIF and other polypeptides cross-reactive with antisera against GAL11, SUG1, SRB2, SRB4, SRB5, and SRB6 proteins. Mediator not only enabled activated transcription but also conferred 8-fold greater activity in basal transcription and 12-fold greater efficiency of phosphorylation of RNA polymerase II by the TFIIH-associated C-terminal repeat domain (CTD) kinase, indicative of mediator-CTD interaction. A holoenzyme form of RNA polymerase II was independently isolated that supported a response to activator proteins with purified basal factors. The holoenzyme proved to consist of mediator associated with core 12-subunit RNA polymerase II.

Original languageEnglish
Pages (from-to)599-608
Number of pages10
JournalCell
Volume77
Issue number4
DOIs
Publication statusPublished - 1994 May 20

Fingerprint

Terminal Repeat Sequences
RNA Polymerase II
Transcriptional Activation
Transcription
Chemical activation
Holoenzymes
Herpes Simplex Virus Protein Vmw65
Peptides
Phosphorylation
Proteins
Yeast
Immune Sera
Phosphotransferases
Yeasts

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kim, Young Joon ; Björklund, Stefan ; Li, Yang ; Sayre, Michael H. ; Kornberg, Roger D. / A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II. In: Cell. 1994 ; Vol. 77, No. 4. pp. 599-608.
@article{7ce821c7366f4376b56530df748936d0,
title = "A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II",
abstract = "A mediator was isolated from yeast that enabled a response to the activator proteins GAL4-VP16 and GCN4 in a transcription system reconstituted with essentially homogeneous basal factors and RNA polymerase II. The mediator comprised some 20 polypeptides, including the three subunits of TFIIF and other polypeptides cross-reactive with antisera against GAL11, SUG1, SRB2, SRB4, SRB5, and SRB6 proteins. Mediator not only enabled activated transcription but also conferred 8-fold greater activity in basal transcription and 12-fold greater efficiency of phosphorylation of RNA polymerase II by the TFIIH-associated C-terminal repeat domain (CTD) kinase, indicative of mediator-CTD interaction. A holoenzyme form of RNA polymerase II was independently isolated that supported a response to activator proteins with purified basal factors. The holoenzyme proved to consist of mediator associated with core 12-subunit RNA polymerase II.",
author = "Kim, {Young Joon} and Stefan Bj{\"o}rklund and Yang Li and Sayre, {Michael H.} and Kornberg, {Roger D.}",
year = "1994",
month = "5",
day = "20",
doi = "10.1016/0092-8674(94)90221-6",
language = "English",
volume = "77",
pages = "599--608",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",

}

A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II. / Kim, Young Joon; Björklund, Stefan; Li, Yang; Sayre, Michael H.; Kornberg, Roger D.

In: Cell, Vol. 77, No. 4, 20.05.1994, p. 599-608.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II

AU - Kim, Young Joon

AU - Björklund, Stefan

AU - Li, Yang

AU - Sayre, Michael H.

AU - Kornberg, Roger D.

PY - 1994/5/20

Y1 - 1994/5/20

N2 - A mediator was isolated from yeast that enabled a response to the activator proteins GAL4-VP16 and GCN4 in a transcription system reconstituted with essentially homogeneous basal factors and RNA polymerase II. The mediator comprised some 20 polypeptides, including the three subunits of TFIIF and other polypeptides cross-reactive with antisera against GAL11, SUG1, SRB2, SRB4, SRB5, and SRB6 proteins. Mediator not only enabled activated transcription but also conferred 8-fold greater activity in basal transcription and 12-fold greater efficiency of phosphorylation of RNA polymerase II by the TFIIH-associated C-terminal repeat domain (CTD) kinase, indicative of mediator-CTD interaction. A holoenzyme form of RNA polymerase II was independently isolated that supported a response to activator proteins with purified basal factors. The holoenzyme proved to consist of mediator associated with core 12-subunit RNA polymerase II.

AB - A mediator was isolated from yeast that enabled a response to the activator proteins GAL4-VP16 and GCN4 in a transcription system reconstituted with essentially homogeneous basal factors and RNA polymerase II. The mediator comprised some 20 polypeptides, including the three subunits of TFIIF and other polypeptides cross-reactive with antisera against GAL11, SUG1, SRB2, SRB4, SRB5, and SRB6 proteins. Mediator not only enabled activated transcription but also conferred 8-fold greater activity in basal transcription and 12-fold greater efficiency of phosphorylation of RNA polymerase II by the TFIIH-associated C-terminal repeat domain (CTD) kinase, indicative of mediator-CTD interaction. A holoenzyme form of RNA polymerase II was independently isolated that supported a response to activator proteins with purified basal factors. The holoenzyme proved to consist of mediator associated with core 12-subunit RNA polymerase II.

UR - http://www.scopus.com/inward/record.url?scp=0028282551&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028282551&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(94)90221-6

DO - 10.1016/0092-8674(94)90221-6

M3 - Article

C2 - 8187178

AN - SCOPUS:0028282551

VL - 77

SP - 599

EP - 608

JO - Cell

JF - Cell

SN - 0092-8674

IS - 4

ER -