A new automated multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) using an AP720S analyzer

Sungsil Lee, Hwan Sub Lim, Jungyong Park, Hyonsuk Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: In the diagnosis of atopic diseases, allergen detection is a crucial step. Multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) is a simple and noninvasive method for in vitro screening of allergen-specific IgE antibodies. Methods: The Korean Inhalant Panel test on 20 patients and Food Panel test on 19 patients were performed using the conventional manual MAST-CLA kit and the new automated MAST-CLA method (automated AP720S system for the Optigen Assay; Hitachi Chemical Diagnostics, Inc., USA) simultaneously. The results were evaluated for positive reactivity and concordance. Results: The results of inhalant panel gave a relatively higher class level result than the food panel. The 8 patients out of 20 (40%) of the inhalation panel, and 9 patients out of 18 (47.4%) of the food panel showed 100% concordance between the 2 systems. Eighteen patients (90%) of the Inhalation Panel and sixteen patients (84.2%) of the Food Panel showed more than 91% concordance. Conclusions: These results suggest that the MAST-CLA assay using the new, automated AP720S analyzer performs well, showing a high concordance rate with conventional MAST-CLA. Compared to manual MAST-CLA, the automated AP720S system has a shorter assay time and uses a smaller serum volume (500 μl) along with other conveniences.

Original languageEnglish
Pages (from-to)182-188
Number of pages7
JournalClinica Chimica Acta
Volume402
Issue number1-2
DOIs
Publication statusPublished - 2009 Apr 1

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Luminescent Measurements
Allergens
Assays
Food
Inhalation
Immunoglobulin E
Screening
Antibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

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title = "A new automated multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) using an AP720S analyzer",
abstract = "Background: In the diagnosis of atopic diseases, allergen detection is a crucial step. Multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) is a simple and noninvasive method for in vitro screening of allergen-specific IgE antibodies. Methods: The Korean Inhalant Panel test on 20 patients and Food Panel test on 19 patients were performed using the conventional manual MAST-CLA kit and the new automated MAST-CLA method (automated AP720S system for the Optigen Assay; Hitachi Chemical Diagnostics, Inc., USA) simultaneously. The results were evaluated for positive reactivity and concordance. Results: The results of inhalant panel gave a relatively higher class level result than the food panel. The 8 patients out of 20 (40{\%}) of the inhalation panel, and 9 patients out of 18 (47.4{\%}) of the food panel showed 100{\%} concordance between the 2 systems. Eighteen patients (90{\%}) of the Inhalation Panel and sixteen patients (84.2{\%}) of the Food Panel showed more than 91{\%} concordance. Conclusions: These results suggest that the MAST-CLA assay using the new, automated AP720S analyzer performs well, showing a high concordance rate with conventional MAST-CLA. Compared to manual MAST-CLA, the automated AP720S system has a shorter assay time and uses a smaller serum volume (500 μl) along with other conveniences.",
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A new automated multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) using an AP720S analyzer. / Lee, Sungsil; Lim, Hwan Sub; Park, Jungyong; Kim, Hyonsuk.

In: Clinica Chimica Acta, Vol. 402, No. 1-2, 01.04.2009, p. 182-188.

Research output: Contribution to journalArticle

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