A novel approach to quantitate protein expression differences in human hepatocellular carcinoma

Kang Sik Park, Hoguen Kim, Yong Ki Paik, Jason N. Marchese, Brian Williamson, Peter Juhasz, Dale Patterson, Steve Martin, Vince C. Gao

Research output: Contribution to conferencePaper

Abstract

An industrial-scale platform based on a platform composed of a Applied Biosystems ICATTM reagent, MDLC purification and tandem mass spectrometry systems to quantitate differential protein expression in normal and cancerous human liver tissue was described. The normal and cancerous human liver tissue were diced from the same patient and the buffered tissues were homogenized, sonicated and ultracentrifuged. The automated MDLC system was used for the separation and collection of the digested peptides. The results show that the platform offers much higher throughput and richer information content for quantitative protein analysis in complicated biological samples.

Original languageEnglish
Pages317-318
Number of pages2
Publication statusPublished - 2002 Dec 1
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2002 Jun 22002 Jun 6

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period02/6/202/6/6

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Tissue
Liver
Proteins
Purification
Mass spectrometry
Throughput
Peptides

All Science Journal Classification (ASJC) codes

  • Spectroscopy

Cite this

Park, K. S., Kim, H., Paik, Y. K., Marchese, J. N., Williamson, B., Juhasz, P., ... Gao, V. C. (2002). A novel approach to quantitate protein expression differences in human hepatocellular carcinoma. 317-318. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.
Park, Kang Sik ; Kim, Hoguen ; Paik, Yong Ki ; Marchese, Jason N. ; Williamson, Brian ; Juhasz, Peter ; Patterson, Dale ; Martin, Steve ; Gao, Vince C. / A novel approach to quantitate protein expression differences in human hepatocellular carcinoma. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.2 p.
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Park, KS, Kim, H, Paik, YK, Marchese, JN, Williamson, B, Juhasz, P, Patterson, D, Martin, S & Gao, VC 2002, 'A novel approach to quantitate protein expression differences in human hepatocellular carcinoma', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 02/6/2 - 02/6/6 pp. 317-318.

A novel approach to quantitate protein expression differences in human hepatocellular carcinoma. / Park, Kang Sik; Kim, Hoguen; Paik, Yong Ki; Marchese, Jason N.; Williamson, Brian; Juhasz, Peter; Patterson, Dale; Martin, Steve; Gao, Vince C.

2002. 317-318 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conferencePaper

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Park KS, Kim H, Paik YK, Marchese JN, Williamson B, Juhasz P et al. A novel approach to quantitate protein expression differences in human hepatocellular carcinoma. 2002. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.