A novel paper MAP method for rapid high resolution histological analysis

Mirae Lee; Jiwon Woo; Doh Hee Kim; Yu Mi Yang; Eunice Yoojin Lee; Jung Hee Kim; Seok Gu Kang; Jin Kyung Shim; Jeong Yoon Park

doi:10.1038/s41598-021-02632-1

A novel paper MAP method for rapid high resolution histological analysis

Mirae Lee, Jiwon Woo, Doh Hee Kim, Yu Mi Yang, Eunice Yoojin Lee, Jung Hee Kim, Seok Gu Kang, Jin Kyung Shim, Jeong Yoon Park

Department of Neurosurgery

Research output: Contribution to journal › Article › peer-review

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Abstract

Three-dimensional visualization of cellular and subcellular-structures in histological-tissues is essential for understanding the complexities of biological-phenomena, especially with regards structural and spatial relationships and pathologlical-diagnosis. Recent advancements in tissue-clearing technology, such as Magnified Analysis of Proteome (MAP), have significantly improved our ability to study biological-structures in three-dimensional space; however, their wide applicability to a variety of tissues is limited by long incubation-times and a need for advanced imaging-systems that are not readily available in most-laboratories. Here, we present optimized MAP-based method for paper-thin samples, Paper-MAP, which allow for rapid clearing and subsequent imaging of three-dimensional sections derived from various tissues using conventional confocal-microscopy. Paper-MAP successfully clear tissues within 1-day, compared to the original-MAP, without significant differences in achieved optical-transparency. As a proof-of-concept, we investigated the vasculature and neuronal-networks of a variety of human and rodent tissues processed via Paper-MAP, in both healthy and diseased contexts, including Alzheimer’s disease and glioma.

Original language	English
Article number	23340
Journal	Scientific reports
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-02632-1
Publication status	Published - 2021 Dec

Bibliographical note

Funding Information:
This work was supported by the Brain Korea 21 PLUS Project for Medical Science, Yonsei University. This study was supported by the Research Grant from Gangnam Severance Hospital, Yonsei University College of Medicine (2020). This study was supported by a faculty research grant of Yonsei University College of Medicine (6-2016-0148, and 6-2020-0109). This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (Grant Number : HI17C1260). In addition, this work was supported by a grant from the National Research Foundation of Korea (NRF-2017R1D1A1B03029560, and NRF-2020R1F1A1072307). This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2020R1A6A3A01097969). This study was supported by the research fund from the Research Institute of Seoul Medical Center (Grant #17-C20). We thank Prof. Jae-Yol Lim and Dr. Yeo-Jun Yoon at Yonsei University College of Medicine for their helpful comments and support on this manuscript.

Publisher Copyright:
© 2021, The Author(s).

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10.1038/s41598-021-02632-1

Cite this

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title = "A novel paper MAP method for rapid high resolution histological analysis",

abstract = "Three-dimensional visualization of cellular and subcellular-structures in histological-tissues is essential for understanding the complexities of biological-phenomena, especially with regards structural and spatial relationships and pathologlical-diagnosis. Recent advancements in tissue-clearing technology, such as Magnified Analysis of Proteome (MAP), have significantly improved our ability to study biological-structures in three-dimensional space; however, their wide applicability to a variety of tissues is limited by long incubation-times and a need for advanced imaging-systems that are not readily available in most-laboratories. Here, we present optimized MAP-based method for paper-thin samples, Paper-MAP, which allow for rapid clearing and subsequent imaging of three-dimensional sections derived from various tissues using conventional confocal-microscopy. Paper-MAP successfully clear tissues within 1-day, compared to the original-MAP, without significant differences in achieved optical-transparency. As a proof-of-concept, we investigated the vasculature and neuronal-networks of a variety of human and rodent tissues processed via Paper-MAP, in both healthy and diseased contexts, including Alzheimer{\textquoteright}s disease and glioma.",

author = "Mirae Lee and Jiwon Woo and Kim, {Doh Hee} and Yang, {Yu Mi} and Lee, {Eunice Yoojin} and Kim, {Jung Hee} and Kang, {Seok Gu} and Shim, {Jin Kyung} and Park, {Jeong Yoon}",

note = "Funding Information: This work was supported by the Brain Korea 21 PLUS Project for Medical Science, Yonsei University. This study was supported by the Research Grant from Gangnam Severance Hospital, Yonsei University College of Medicine (2020). This study was supported by a faculty research grant of Yonsei University College of Medicine (6-2016-0148, and 6-2020-0109). This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (Grant Number : HI17C1260). In addition, this work was supported by a grant from the National Research Foundation of Korea (NRF-2017R1D1A1B03029560, and NRF-2020R1F1A1072307). This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2020R1A6A3A01097969). This study was supported by the research fund from the Research Institute of Seoul Medical Center (Grant #17-C20). We thank Prof. Jae-Yol Lim and Dr. Yeo-Jun Yoon at Yonsei University College of Medicine for their helpful comments and support on this manuscript. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = dec,

doi = "10.1038/s41598-021-02632-1",

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AU - Woo, Jiwon

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AU - Yang, Yu Mi

AU - Lee, Eunice Yoojin

AU - Kim, Jung Hee

AU - Kang, Seok Gu

AU - Shim, Jin Kyung

AU - Park, Jeong Yoon

N1 - Funding Information: This work was supported by the Brain Korea 21 PLUS Project for Medical Science, Yonsei University. This study was supported by the Research Grant from Gangnam Severance Hospital, Yonsei University College of Medicine (2020). This study was supported by a faculty research grant of Yonsei University College of Medicine (6-2016-0148, and 6-2020-0109). This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (Grant Number : HI17C1260). In addition, this work was supported by a grant from the National Research Foundation of Korea (NRF-2017R1D1A1B03029560, and NRF-2020R1F1A1072307). This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2020R1A6A3A01097969). This study was supported by the research fund from the Research Institute of Seoul Medical Center (Grant #17-C20). We thank Prof. Jae-Yol Lim and Dr. Yeo-Jun Yoon at Yonsei University College of Medicine for their helpful comments and support on this manuscript. Publisher Copyright: © 2021, The Author(s).

PY - 2021/12

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N2 - Three-dimensional visualization of cellular and subcellular-structures in histological-tissues is essential for understanding the complexities of biological-phenomena, especially with regards structural and spatial relationships and pathologlical-diagnosis. Recent advancements in tissue-clearing technology, such as Magnified Analysis of Proteome (MAP), have significantly improved our ability to study biological-structures in three-dimensional space; however, their wide applicability to a variety of tissues is limited by long incubation-times and a need for advanced imaging-systems that are not readily available in most-laboratories. Here, we present optimized MAP-based method for paper-thin samples, Paper-MAP, which allow for rapid clearing and subsequent imaging of three-dimensional sections derived from various tissues using conventional confocal-microscopy. Paper-MAP successfully clear tissues within 1-day, compared to the original-MAP, without significant differences in achieved optical-transparency. As a proof-of-concept, we investigated the vasculature and neuronal-networks of a variety of human and rodent tissues processed via Paper-MAP, in both healthy and diseased contexts, including Alzheimer’s disease and glioma.

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A novel paper MAP method for rapid high resolution histological analysis

Abstract

Bibliographical note

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