A novel parvovirus isolated from Manchurian chipmunks

Byung Chul Yoo, Dong Ho Lee, Sill Moo Park, Joong Won Park, Chung Yong Kim, Hyo Suk Lee, Jeong Sun Seo, Kie Jung Park, Wang-Shick Ryu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A novel parvovirus was identified in Manchurian chipmunks inhabiting Korea, Hepatitis B surface antigen (HBsAg) was detected in sera from 4 animals among 62 apparently healthy chipmunks, Electron microscopic examination of the HBsAg-positive sera revealed virus-like spherical particles 20-22 nm in diameter. Extraction of nucleic acid under annealing conditions from the serum samples containing virus-like particles yielded a single species of DNA molecule with the electrophoretic mobility of 5.6-kb double-stranded DNA. Four overlapping clones that encompassed almost the full-length viral genome, except both ends, were obtained. By sequencing these clones, we determined the sequence of 5097 nucleotides of the viral DNA. Two open reading frames were identified, with the left side open reading frame encoding a putative nonstructural protein and the right side open reading frame encoding a putative capsid protein. The nucleotide and amino acid sequences showed significant homology to parvovirus B19 and simian parvovirus, but showed little homology to other mammalian autonomous parvoviruses or adeno-associated viruses. These observations indicate that the virus isolated from Manchurian chipmunks is a novel parvovirus and may be a potentially useful animal model of human B19 infection as a new member of the Erythrovirus genus of the Parvoviridae.

Original languageEnglish
Pages (from-to)250-258
Number of pages9
JournalVirology
Volume253
Issue number2
DOIs
Publication statusPublished - 1999 Jan 20

Fingerprint

Sciuridae
Parvovirus
Erythrovirus
Open Reading Frames
Hepatitis B Surface Antigens
Parvoviridae
Clone Cells
Serum
Viruses
Dependovirus
Viral Genome
DNA
Viral DNA
Capsid Proteins
Korea
Virion
Nucleic Acids
Amino Acid Sequence
Nucleotides
Animal Models

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Yoo, B. C., Lee, D. H., Park, S. M., Park, J. W., Kim, C. Y., Lee, H. S., ... Ryu, W-S. (1999). A novel parvovirus isolated from Manchurian chipmunks. Virology, 253(2), 250-258. https://doi.org/10.1006/viro.1998.9518
Yoo, Byung Chul ; Lee, Dong Ho ; Park, Sill Moo ; Park, Joong Won ; Kim, Chung Yong ; Lee, Hyo Suk ; Seo, Jeong Sun ; Park, Kie Jung ; Ryu, Wang-Shick. / A novel parvovirus isolated from Manchurian chipmunks. In: Virology. 1999 ; Vol. 253, No. 2. pp. 250-258.
@article{871d446cf69e4473b07a5c44c4318e11,
title = "A novel parvovirus isolated from Manchurian chipmunks",
abstract = "A novel parvovirus was identified in Manchurian chipmunks inhabiting Korea, Hepatitis B surface antigen (HBsAg) was detected in sera from 4 animals among 62 apparently healthy chipmunks, Electron microscopic examination of the HBsAg-positive sera revealed virus-like spherical particles 20-22 nm in diameter. Extraction of nucleic acid under annealing conditions from the serum samples containing virus-like particles yielded a single species of DNA molecule with the electrophoretic mobility of 5.6-kb double-stranded DNA. Four overlapping clones that encompassed almost the full-length viral genome, except both ends, were obtained. By sequencing these clones, we determined the sequence of 5097 nucleotides of the viral DNA. Two open reading frames were identified, with the left side open reading frame encoding a putative nonstructural protein and the right side open reading frame encoding a putative capsid protein. The nucleotide and amino acid sequences showed significant homology to parvovirus B19 and simian parvovirus, but showed little homology to other mammalian autonomous parvoviruses or adeno-associated viruses. These observations indicate that the virus isolated from Manchurian chipmunks is a novel parvovirus and may be a potentially useful animal model of human B19 infection as a new member of the Erythrovirus genus of the Parvoviridae.",
author = "Yoo, {Byung Chul} and Lee, {Dong Ho} and Park, {Sill Moo} and Park, {Joong Won} and Kim, {Chung Yong} and Lee, {Hyo Suk} and Seo, {Jeong Sun} and Park, {Kie Jung} and Wang-Shick Ryu",
year = "1999",
month = "1",
day = "20",
doi = "10.1006/viro.1998.9518",
language = "English",
volume = "253",
pages = "250--258",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

Yoo, BC, Lee, DH, Park, SM, Park, JW, Kim, CY, Lee, HS, Seo, JS, Park, KJ & Ryu, W-S 1999, 'A novel parvovirus isolated from Manchurian chipmunks', Virology, vol. 253, no. 2, pp. 250-258. https://doi.org/10.1006/viro.1998.9518

A novel parvovirus isolated from Manchurian chipmunks. / Yoo, Byung Chul; Lee, Dong Ho; Park, Sill Moo; Park, Joong Won; Kim, Chung Yong; Lee, Hyo Suk; Seo, Jeong Sun; Park, Kie Jung; Ryu, Wang-Shick.

In: Virology, Vol. 253, No. 2, 20.01.1999, p. 250-258.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A novel parvovirus isolated from Manchurian chipmunks

AU - Yoo, Byung Chul

AU - Lee, Dong Ho

AU - Park, Sill Moo

AU - Park, Joong Won

AU - Kim, Chung Yong

AU - Lee, Hyo Suk

AU - Seo, Jeong Sun

AU - Park, Kie Jung

AU - Ryu, Wang-Shick

PY - 1999/1/20

Y1 - 1999/1/20

N2 - A novel parvovirus was identified in Manchurian chipmunks inhabiting Korea, Hepatitis B surface antigen (HBsAg) was detected in sera from 4 animals among 62 apparently healthy chipmunks, Electron microscopic examination of the HBsAg-positive sera revealed virus-like spherical particles 20-22 nm in diameter. Extraction of nucleic acid under annealing conditions from the serum samples containing virus-like particles yielded a single species of DNA molecule with the electrophoretic mobility of 5.6-kb double-stranded DNA. Four overlapping clones that encompassed almost the full-length viral genome, except both ends, were obtained. By sequencing these clones, we determined the sequence of 5097 nucleotides of the viral DNA. Two open reading frames were identified, with the left side open reading frame encoding a putative nonstructural protein and the right side open reading frame encoding a putative capsid protein. The nucleotide and amino acid sequences showed significant homology to parvovirus B19 and simian parvovirus, but showed little homology to other mammalian autonomous parvoviruses or adeno-associated viruses. These observations indicate that the virus isolated from Manchurian chipmunks is a novel parvovirus and may be a potentially useful animal model of human B19 infection as a new member of the Erythrovirus genus of the Parvoviridae.

AB - A novel parvovirus was identified in Manchurian chipmunks inhabiting Korea, Hepatitis B surface antigen (HBsAg) was detected in sera from 4 animals among 62 apparently healthy chipmunks, Electron microscopic examination of the HBsAg-positive sera revealed virus-like spherical particles 20-22 nm in diameter. Extraction of nucleic acid under annealing conditions from the serum samples containing virus-like particles yielded a single species of DNA molecule with the electrophoretic mobility of 5.6-kb double-stranded DNA. Four overlapping clones that encompassed almost the full-length viral genome, except both ends, were obtained. By sequencing these clones, we determined the sequence of 5097 nucleotides of the viral DNA. Two open reading frames were identified, with the left side open reading frame encoding a putative nonstructural protein and the right side open reading frame encoding a putative capsid protein. The nucleotide and amino acid sequences showed significant homology to parvovirus B19 and simian parvovirus, but showed little homology to other mammalian autonomous parvoviruses or adeno-associated viruses. These observations indicate that the virus isolated from Manchurian chipmunks is a novel parvovirus and may be a potentially useful animal model of human B19 infection as a new member of the Erythrovirus genus of the Parvoviridae.

UR - http://www.scopus.com/inward/record.url?scp=0033585369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033585369&partnerID=8YFLogxK

U2 - 10.1006/viro.1998.9518

DO - 10.1006/viro.1998.9518

M3 - Article

C2 - 9918883

AN - SCOPUS:0033585369

VL - 253

SP - 250

EP - 258

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -

Yoo BC, Lee DH, Park SM, Park JW, Kim CY, Lee HS et al. A novel parvovirus isolated from Manchurian chipmunks. Virology. 1999 Jan 20;253(2):250-258. https://doi.org/10.1006/viro.1998.9518