A novel peptide, nicotinyl–isoleucine–valine–histidine (Na–IVH), promotes antioxidant gene expression and wound healing in HaCaT cells

Dong Hwee Son; Dong Joo Yang; Ji Su Sun; Seul Ki Kim; Namju Kang; Jung Yun Kang; Yun Hee Choi; Jeong Hun Lee; Sang Hyun Moh; Dong Min Shin; Ki Woo Kim

doi:10.3390/md16080262

A novel peptide, nicotinyl–isoleucine–valine–histidine (Na–IVH), promotes antioxidant gene expression and wound healing in HaCaT cells

Dong Hwee Son, Dong Joo Yang, Ji Su Sun, Seul Ki Kim, Namju Kang, Jung Yun Kang, Yun Hee Choi, Jeong Hun Lee, Sang Hyun Moh, Dong Min Shin, Ki Woo Kim

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12 Citations (Scopus)

Abstract

Nicotinamide (NA), a water-soluble Vitamin B₃, has been shown to exert cellular-protective effects against reactive oxygen species (ROS). In order to improve the cellular-protective effects of NA, we synthesized a novel compound, nicotinyl–isoleucine–valine–histidine (NA–IVH), by combining NA with jellyfish peptides’ IVH. In the present study, we examined the cellular-protective effects of the novel synthetic nicotinyl-peptide, NA–IVH. We found that NA–IVH enhances the radical scavenging activity with a robust increase of the nuclear factor (erythroid-derived 2)-like factor (Nrf2) expression in human HaCaT keratinocytes. In addition, NA–IVH protected the cells from hydrogen peroxide (H₂O₂)-induced cell death. Interestingly, NA–IVH exhibited an improved wound-healing effect in a high glucose condition, possibly through the regulation of reactive oxygen species (ROS). Collectively, our results imply that a novel nicotinyl-peptide, NA–IVH, has a wound-healing effect in a hyperglycemic condition, possibly by modulating excessive ROS.

Original language	English
Article number	262
Journal	Marine Drugs
Volume	16
Issue number	8
DOIs	https://doi.org/10.3390/md16080262
Publication status	Published - 2018 Aug 1

Bibliographical note

Funding Information:
Funding: This research was funded by the National Research Foundation, grant numbers [2016R1C1B3012748 and 2016R1A5A2008630], and the Korea Health Industry Development Institute, grant number [HI17C0745] for K.W.K. The research also was supported by National Research Foundation, grant numbers [2016R1A5A2008630] for D.M.S.

Funding Information:
Acknowledgments: We would like to thank Ann W. Kinyua (Yonsei University) for the critical reading of this manuscript. This study was partly supported by the 2015 Ildong Foodis Research Grant from Korean Society for the Study of Obesity.

Funding Information:
This research was funded by the National Research Foundation, grant numbers [2016R1C1B3012748 and 2016R1A5A2008630], and the Korea Health Industry Development Institute, grant number [HI17C0745] for K.W.K. The research also was supported by National Research Foundation, grant numbers [2016R1A5A2008630] for D.M.S.

Publisher Copyright:
© 2018 by the authors.

All Science Journal Classification (ASJC) codes

Drug Discovery
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Pharmaceutical Science

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@article{90f7240cf7dc4f8683507f9ca89cf4d8,

title = "A novel peptide, nicotinyl–isoleucine–valine–histidine (Na–IVH), promotes antioxidant gene expression and wound healing in HaCaT cells",

abstract = "Nicotinamide (NA), a water-soluble Vitamin B3, has been shown to exert cellular-protective effects against reactive oxygen species (ROS). In order to improve the cellular-protective effects of NA, we synthesized a novel compound, nicotinyl–isoleucine–valine–histidine (NA–IVH), by combining NA with jellyfish peptides{\textquoteright} IVH. In the present study, we examined the cellular-protective effects of the novel synthetic nicotinyl-peptide, NA–IVH. We found that NA–IVH enhances the radical scavenging activity with a robust increase of the nuclear factor (erythroid-derived 2)-like factor (Nrf2) expression in human HaCaT keratinocytes. In addition, NA–IVH protected the cells from hydrogen peroxide (H2O2)-induced cell death. Interestingly, NA–IVH exhibited an improved wound-healing effect in a high glucose condition, possibly through the regulation of reactive oxygen species (ROS). Collectively, our results imply that a novel nicotinyl-peptide, NA–IVH, has a wound-healing effect in a hyperglycemic condition, possibly by modulating excessive ROS.",

author = "Son, {Dong Hwee} and Yang, {Dong Joo} and Sun, {Ji Su} and Kim, {Seul Ki} and Namju Kang and Kang, {Jung Yun} and Choi, {Yun Hee} and Lee, {Jeong Hun} and Moh, {Sang Hyun} and Shin, {Dong Min} and Kim, {Ki Woo}",

note = "Funding Information: Funding: This research was funded by the National Research Foundation, grant numbers [2016R1C1B3012748 and 2016R1A5A2008630], and the Korea Health Industry Development Institute, grant number [HI17C0745] for K.W.K. The research also was supported by National Research Foundation, grant numbers [2016R1A5A2008630] for D.M.S. Funding Information: Acknowledgments: We would like to thank Ann W. Kinyua (Yonsei University) for the critical reading of this manuscript. This study was partly supported by the 2015 Ildong Foodis Research Grant from Korean Society for the Study of Obesity. Funding Information: This research was funded by the National Research Foundation, grant numbers [2016R1C1B3012748 and 2016R1A5A2008630], and the Korea Health Industry Development Institute, grant number [HI17C0745] for K.W.K. The research also was supported by National Research Foundation, grant numbers [2016R1A5A2008630] for D.M.S. Publisher Copyright: {\textcopyright} 2018 by the authors.",

year = "2018",

month = aug,

day = "1",

doi = "10.3390/md16080262",

language = "English",

volume = "16",

journal = "Marine Drugs",

issn = "1660-3397",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "8",

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T1 - A novel peptide, nicotinyl–isoleucine–valine–histidine (Na–IVH), promotes antioxidant gene expression and wound healing in HaCaT cells

AU - Son, Dong Hwee

AU - Yang, Dong Joo

AU - Sun, Ji Su

AU - Kim, Seul Ki

AU - Kang, Namju

AU - Kang, Jung Yun

AU - Choi, Yun Hee

AU - Lee, Jeong Hun

AU - Moh, Sang Hyun

AU - Shin, Dong Min

AU - Kim, Ki Woo

N1 - Funding Information: Funding: This research was funded by the National Research Foundation, grant numbers [2016R1C1B3012748 and 2016R1A5A2008630], and the Korea Health Industry Development Institute, grant number [HI17C0745] for K.W.K. The research also was supported by National Research Foundation, grant numbers [2016R1A5A2008630] for D.M.S. Funding Information: Acknowledgments: We would like to thank Ann W. Kinyua (Yonsei University) for the critical reading of this manuscript. This study was partly supported by the 2015 Ildong Foodis Research Grant from Korean Society for the Study of Obesity. Funding Information: This research was funded by the National Research Foundation, grant numbers [2016R1C1B3012748 and 2016R1A5A2008630], and the Korea Health Industry Development Institute, grant number [HI17C0745] for K.W.K. The research also was supported by National Research Foundation, grant numbers [2016R1A5A2008630] for D.M.S. Publisher Copyright: © 2018 by the authors.

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Nicotinamide (NA), a water-soluble Vitamin B3, has been shown to exert cellular-protective effects against reactive oxygen species (ROS). In order to improve the cellular-protective effects of NA, we synthesized a novel compound, nicotinyl–isoleucine–valine–histidine (NA–IVH), by combining NA with jellyfish peptides’ IVH. In the present study, we examined the cellular-protective effects of the novel synthetic nicotinyl-peptide, NA–IVH. We found that NA–IVH enhances the radical scavenging activity with a robust increase of the nuclear factor (erythroid-derived 2)-like factor (Nrf2) expression in human HaCaT keratinocytes. In addition, NA–IVH protected the cells from hydrogen peroxide (H2O2)-induced cell death. Interestingly, NA–IVH exhibited an improved wound-healing effect in a high glucose condition, possibly through the regulation of reactive oxygen species (ROS). Collectively, our results imply that a novel nicotinyl-peptide, NA–IVH, has a wound-healing effect in a hyperglycemic condition, possibly by modulating excessive ROS.

AB - Nicotinamide (NA), a water-soluble Vitamin B3, has been shown to exert cellular-protective effects against reactive oxygen species (ROS). In order to improve the cellular-protective effects of NA, we synthesized a novel compound, nicotinyl–isoleucine–valine–histidine (NA–IVH), by combining NA with jellyfish peptides’ IVH. In the present study, we examined the cellular-protective effects of the novel synthetic nicotinyl-peptide, NA–IVH. We found that NA–IVH enhances the radical scavenging activity with a robust increase of the nuclear factor (erythroid-derived 2)-like factor (Nrf2) expression in human HaCaT keratinocytes. In addition, NA–IVH protected the cells from hydrogen peroxide (H2O2)-induced cell death. Interestingly, NA–IVH exhibited an improved wound-healing effect in a high glucose condition, possibly through the regulation of reactive oxygen species (ROS). Collectively, our results imply that a novel nicotinyl-peptide, NA–IVH, has a wound-healing effect in a hyperglycemic condition, possibly by modulating excessive ROS.

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DO - 10.3390/md16080262

M3 - Article

C2 - 30071627

AN - SCOPUS:85053720050

SN - 1660-3397

VL - 16

JO - Marine Drugs

JF - Marine Drugs

IS - 8

M1 - 262

ER -

A novel peptide, nicotinyl–isoleucine–valine–histidine (Na–IVH), promotes antioxidant gene expression and wound healing in HaCaT cells

Abstract

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