A novel recombinant basic fibroblast growth factor and its secretion

Young Doug Sohn, Hyun Joung Lim, Ki Chul Hwang, Jun Hye Kwon, Hyun Young Park, Kwang Hoe Chung, Seung Yun Cho, Yangsoo Jang

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Abstract

Basic fibroblast growth factor (FGF-2) is a pleiotropic mitogen which plays an important role in cell growth, differentiation, migration, and survival in different cells and organ systems. Recently, several clinical applications for FGF-2 gene transfer are being evaluated in wound healing and collateral artery development to relieve myocardial and peripheral ischemia due to the ability of FGF-2 to regulate the growth and function of vascular cells. However, FGF-2 lacks a classical hydrophobic secretion signal peptide, the FGF-2 chimeras containing various signal sequences have been explored. In this study, a novel recombinant 4sFGF-2 was constructed by replacing nine residues from the amino-terminus of native FGF-2 (Met1 to Leu9) with eight amino acid residues of signal peptide of FGF-4 (Met1 to Ala8) to better increase the secretion level of FGF-2. When the recombinant FGF-2 gene, cloned into the expression vector with CMV promoter, was expressed in COS-7 cells, the recombinant 4sFGF-2 was highly secreted into the media. The secreted 4sFGF-2 showed the same biological activity as the native FGF-2 in the dose-response effects on DNA synthesis and cell growth of rat aortic smooth muscle cells (RASMCs) and NIH3T3 cells. The 4sFGF-2 also was able to activate MAPK as wild FGF-2 in RASMCs. These results indicate that a novel recombinant 4sFGF-2 may be useful as clinical applicability of angiogenic growth factor gene transfer.

Original languageEnglish
Pages (from-to)931-936
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume284
Issue number4
DOIs
Publication statusPublished - 2001 Jan 1

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sohn, Y. D., Lim, H. J., Hwang, K. C., Kwon, J. H., Park, H. Y., Chung, K. H., Cho, S. Y., & Jang, Y. (2001). A novel recombinant basic fibroblast growth factor and its secretion. Biochemical and Biophysical Research Communications, 284(4), 931-936. https://doi.org/10.1006/bbrc.2001.5076