A novel single-step fabrication technique to create heterogeneous poly(ethylene glycol) hydrogel microstructures containing multiple phenotypes of mammalian cells

Jeanna C. Zguris, Laura J. Itle, Won-Gun Koh, Michael V. Pishko

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In this study, a novel method for the one-step fabrication of stacked hydrogel microstructures using a microfluidic mold is presented. The fabrication of these structures takes advantage of the laminar flow regime in microfluidic devices, limiting the mixing of polymer precursor solutions. To create multilayered hydrogel structures, microfluidic devices were rotated 90° from the traditional xy axes and sealed with a cover slip. Two discreet fluidic regions form in the channels, resulting in the multilayered hydrogel upon UV polymerization. Multilayered patterned poly(ethylene glycol) hydrogel arrays (60 μm tall, 250 μm wide) containing fluorescent dyes, fluorescein isothiocyanate, and tetramethylrhodamine isothiocyanate were created for imaging purposes. Additionally, this method Was used to generate hydrogel layers containing murine fibroblasts and macrophages. The cell adhesion promoter, RGD, was added to hydrogel precursor solution to enhance fibroblast cell spreading within the hydrogel matrix in one layer, but not the other. We were able to successfully generate patterns of hydrogels containing multiple phenotypes by using this technique.

Original languageEnglish
Pages (from-to)4168-4174
Number of pages7
JournalLangmuir
Volume21
Issue number9
DOIs
Publication statusPublished - 2005 Apr 26

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phenotype
microfluidic devices
Hydrogel
fibroblasts
Hydrogels
Polyethylene glycols
glycols
ethylene
Cells
Fabrication
microstructure
Microstructure
fabrication
macrophages
fluidics
laminar flow
Microfluidics
adhesion
slip
polymerization

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

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abstract = "In this study, a novel method for the one-step fabrication of stacked hydrogel microstructures using a microfluidic mold is presented. The fabrication of these structures takes advantage of the laminar flow regime in microfluidic devices, limiting the mixing of polymer precursor solutions. To create multilayered hydrogel structures, microfluidic devices were rotated 90° from the traditional xy axes and sealed with a cover slip. Two discreet fluidic regions form in the channels, resulting in the multilayered hydrogel upon UV polymerization. Multilayered patterned poly(ethylene glycol) hydrogel arrays (60 μm tall, 250 μm wide) containing fluorescent dyes, fluorescein isothiocyanate, and tetramethylrhodamine isothiocyanate were created for imaging purposes. Additionally, this method Was used to generate hydrogel layers containing murine fibroblasts and macrophages. The cell adhesion promoter, RGD, was added to hydrogel precursor solution to enhance fibroblast cell spreading within the hydrogel matrix in one layer, but not the other. We were able to successfully generate patterns of hydrogels containing multiple phenotypes by using this technique.",
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A novel single-step fabrication technique to create heterogeneous poly(ethylene glycol) hydrogel microstructures containing multiple phenotypes of mammalian cells. / Zguris, Jeanna C.; Itle, Laura J.; Koh, Won-Gun; Pishko, Michael V.

In: Langmuir, Vol. 21, No. 9, 26.04.2005, p. 4168-4174.

Research output: Contribution to journalArticle

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