A novel uracil-DNA glycosylase family related to the helix-hairpin-helix DNA glycosylase superfamily

Ji Hyung Chung, Eun Kyoung Im, Hyun Young Park, Jun Hye Kwon, Seahyoung Lee, Jaewon Oh, Ki Chul Hwang, Jong Ho Lee, Yangsoo Jang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Cytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase (UDG), a ubiquitous uracil-excising enzyme found in bacteria and eukaryotes, is one of the enzymes that repair this kind of DNA damage. To date, no UDG-coding gene has been identified in Methanococcus jannaschii, although its entire genome was deciphered. Here, we have identified and characterized a novel UDG from M.jannaschii designated as MjUDG. It efficiently removed uracil from both single- and double-stranded DNA. MjUDG also catalyzes the excision of 8-oxoguanine from DNA. MjUDG has a helix-hairpin-helix motif and a [4Fe-4S]-binding cluster that is considered to be important for the DNA binding and catalytic activity. Although MjUDG shares these features with other structural families such as endonuclease III and mismatch-specific DNA glycosylase (MIG), unique conserved amino acids and substrate specificity distinguish MjUDG from other families. Also, a homologous member of MjUDG was identified in Aquifex aeolicus. We report that MjUDG belongs to a novel UDG family that has not been described to date.

Original languageEnglish
Pages (from-to)2045-2055
Number of pages11
JournalNucleic acids research
Volume31
Issue number8
DOIs
Publication statusPublished - 2003 Apr 15

All Science Journal Classification (ASJC) codes

  • Genetics

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