Cuticle formation and molting are critical for the development of Caenorhabditis elegans. To understand cuticle formation more clearly, we screened for suppressors in transgenic worms that expressed dominant ROL-6 collagen proteins. The suro-1 mutant, which is mild dumpy, exhibited a different ROL-6::GFP localization pattern compared to other Dpy mutants. We identified mutations in three suro-1 mutants, and found that suro-1 (ORF R11A5.7) encodes a putative zinc-carboxypeptidase homologue. The expression of this enzyme in the hypodermis and the genetic interactions between this enzyme and other collagen-modifying enzyme mutants suggest a regulatory role in collagen processing and cuticle organization for this novel carboxypeptidase. These findings aid our understanding of cuticle formation during worm development.
Bibliographical noteFunding Information:
This work was supported by research Grants from the National Cancer Center ( NCC-0810070 ) and the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology ( R0A-2007-000-20011-0 , World Class University Program, R31-2008-000-10086-0 and 2009-0084805 ). Additionally, Y.J.K. is fellowship awardees of the Brain Korea 21 program. We thank the Caenorhabditis Genetics Center (CGC) for providing worm strains, Dr. Mello for distributing the pRF4 plasmid, and Dr. Herbert for the TTX-3::GFP plasmid.
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology
- Cell Biology