A nuclear-encoded chloroplast-targeted S1 RNA-binding domain protein affects chloroplast rRNA processing and is crucial for the normal growth of Arabidopsis thaliana

Ji Hoon Han, Kwanuk Lee, Kwang Ho Lee, Sunyo Jung, Young Jeon, Hyunsook Pai, Hunseung Kang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Summary Despite the fact that a variety of nuclear-encoded RNA-binding proteins (RBPs) are targeted to the chloroplast and play essential roles during post-transcriptional RNA metabolism in the chloroplast, the physiological roles of the majority of chloroplast-targeted RBPs remain elusive. Here, we investigated the functional role of a nuclear-encoded S1 domain-containing RBP, designated SDP, in the growth and development of Arabidopsis thaliana. Confocal analysis of the SDP-green fluorescent protein revealed that SDP was localized to the chloroplast. The loss-of-function sdp mutant displayed retarded seed germination and pale-green phenotypes, and grew smaller than the wild-type plants. Chlorophyll a content and photosynthetic activity of the sdp mutant were much lower than those of wild-type plants, and the structures of the chloroplast and the prolamellar body were abnormal in the sdp mutant. The processing of rRNAs in the chloroplast was defective in the sdp mutant, and SDP was able to bind chloroplast 23S, 16S, 5S and 4.5S rRNAs. Notably, SDP possesses RNA chaperone activity. Transcript levels of the nuclear genes involved in chlorophyll biosynthesis were altered in the sdp mutant. Collectively, these results suggest that chloroplast-targeted SDP harboring RNA chaperone activity affects rRNA processing, chloroplast biogenesis and photosynthetic activity, which is crucial for normal growth of Arabidopsis. Significance Statement The nuclear-encoded chloroplast-targeted S1 domain-containing protein (SDP) harboring RNA chaperone activity affects rRNA processing, chloroplast biogenesis, and photosynthetic activity, which is crucial for normal growth of Arabidopsis.

Original languageEnglish
Pages (from-to)277-289
Number of pages13
JournalPlant Journal
Volume83
Issue number2
DOIs
Publication statusPublished - 2015 Jul 1

Fingerprint

Chloroplasts
Arabidopsis
Arabidopsis thaliana
chloroplasts
ribosomal RNA
RNA
Growth
proteins
RNA-binding proteins
mutants
RNA-Binding Proteins
Chloroplast RNA
RNA Recognition Motif Proteins
nuclear-encoded proteins
Plant Structures
Nuclear RNA
chlorophyll
Chlorophyll
Germination
Green Fluorescent Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Han, Ji Hoon ; Lee, Kwanuk ; Lee, Kwang Ho ; Jung, Sunyo ; Jeon, Young ; Pai, Hyunsook ; Kang, Hunseung. / A nuclear-encoded chloroplast-targeted S1 RNA-binding domain protein affects chloroplast rRNA processing and is crucial for the normal growth of Arabidopsis thaliana. In: Plant Journal. 2015 ; Vol. 83, No. 2. pp. 277-289.
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abstract = "Summary Despite the fact that a variety of nuclear-encoded RNA-binding proteins (RBPs) are targeted to the chloroplast and play essential roles during post-transcriptional RNA metabolism in the chloroplast, the physiological roles of the majority of chloroplast-targeted RBPs remain elusive. Here, we investigated the functional role of a nuclear-encoded S1 domain-containing RBP, designated SDP, in the growth and development of Arabidopsis thaliana. Confocal analysis of the SDP-green fluorescent protein revealed that SDP was localized to the chloroplast. The loss-of-function sdp mutant displayed retarded seed germination and pale-green phenotypes, and grew smaller than the wild-type plants. Chlorophyll a content and photosynthetic activity of the sdp mutant were much lower than those of wild-type plants, and the structures of the chloroplast and the prolamellar body were abnormal in the sdp mutant. The processing of rRNAs in the chloroplast was defective in the sdp mutant, and SDP was able to bind chloroplast 23S, 16S, 5S and 4.5S rRNAs. Notably, SDP possesses RNA chaperone activity. Transcript levels of the nuclear genes involved in chlorophyll biosynthesis were altered in the sdp mutant. Collectively, these results suggest that chloroplast-targeted SDP harboring RNA chaperone activity affects rRNA processing, chloroplast biogenesis and photosynthetic activity, which is crucial for normal growth of Arabidopsis. Significance Statement The nuclear-encoded chloroplast-targeted S1 domain-containing protein (SDP) harboring RNA chaperone activity affects rRNA processing, chloroplast biogenesis, and photosynthetic activity, which is crucial for normal growth of Arabidopsis.",
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A nuclear-encoded chloroplast-targeted S1 RNA-binding domain protein affects chloroplast rRNA processing and is crucial for the normal growth of Arabidopsis thaliana. / Han, Ji Hoon; Lee, Kwanuk; Lee, Kwang Ho; Jung, Sunyo; Jeon, Young; Pai, Hyunsook; Kang, Hunseung.

In: Plant Journal, Vol. 83, No. 2, 01.07.2015, p. 277-289.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A nuclear-encoded chloroplast-targeted S1 RNA-binding domain protein affects chloroplast rRNA processing and is crucial for the normal growth of Arabidopsis thaliana

AU - Han, Ji Hoon

AU - Lee, Kwanuk

AU - Lee, Kwang Ho

AU - Jung, Sunyo

AU - Jeon, Young

AU - Pai, Hyunsook

AU - Kang, Hunseung

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Summary Despite the fact that a variety of nuclear-encoded RNA-binding proteins (RBPs) are targeted to the chloroplast and play essential roles during post-transcriptional RNA metabolism in the chloroplast, the physiological roles of the majority of chloroplast-targeted RBPs remain elusive. Here, we investigated the functional role of a nuclear-encoded S1 domain-containing RBP, designated SDP, in the growth and development of Arabidopsis thaliana. Confocal analysis of the SDP-green fluorescent protein revealed that SDP was localized to the chloroplast. The loss-of-function sdp mutant displayed retarded seed germination and pale-green phenotypes, and grew smaller than the wild-type plants. Chlorophyll a content and photosynthetic activity of the sdp mutant were much lower than those of wild-type plants, and the structures of the chloroplast and the prolamellar body were abnormal in the sdp mutant. The processing of rRNAs in the chloroplast was defective in the sdp mutant, and SDP was able to bind chloroplast 23S, 16S, 5S and 4.5S rRNAs. Notably, SDP possesses RNA chaperone activity. Transcript levels of the nuclear genes involved in chlorophyll biosynthesis were altered in the sdp mutant. Collectively, these results suggest that chloroplast-targeted SDP harboring RNA chaperone activity affects rRNA processing, chloroplast biogenesis and photosynthetic activity, which is crucial for normal growth of Arabidopsis. Significance Statement The nuclear-encoded chloroplast-targeted S1 domain-containing protein (SDP) harboring RNA chaperone activity affects rRNA processing, chloroplast biogenesis, and photosynthetic activity, which is crucial for normal growth of Arabidopsis.

AB - Summary Despite the fact that a variety of nuclear-encoded RNA-binding proteins (RBPs) are targeted to the chloroplast and play essential roles during post-transcriptional RNA metabolism in the chloroplast, the physiological roles of the majority of chloroplast-targeted RBPs remain elusive. Here, we investigated the functional role of a nuclear-encoded S1 domain-containing RBP, designated SDP, in the growth and development of Arabidopsis thaliana. Confocal analysis of the SDP-green fluorescent protein revealed that SDP was localized to the chloroplast. The loss-of-function sdp mutant displayed retarded seed germination and pale-green phenotypes, and grew smaller than the wild-type plants. Chlorophyll a content and photosynthetic activity of the sdp mutant were much lower than those of wild-type plants, and the structures of the chloroplast and the prolamellar body were abnormal in the sdp mutant. The processing of rRNAs in the chloroplast was defective in the sdp mutant, and SDP was able to bind chloroplast 23S, 16S, 5S and 4.5S rRNAs. Notably, SDP possesses RNA chaperone activity. Transcript levels of the nuclear genes involved in chlorophyll biosynthesis were altered in the sdp mutant. Collectively, these results suggest that chloroplast-targeted SDP harboring RNA chaperone activity affects rRNA processing, chloroplast biogenesis and photosynthetic activity, which is crucial for normal growth of Arabidopsis. Significance Statement The nuclear-encoded chloroplast-targeted S1 domain-containing protein (SDP) harboring RNA chaperone activity affects rRNA processing, chloroplast biogenesis, and photosynthetic activity, which is crucial for normal growth of Arabidopsis.

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U2 - 10.1111/tpj.12889

DO - 10.1111/tpj.12889

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SN - 0960-7412

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