A Nudix Hydrolase Protein, Ysa1, Regulates Oxidative Stress Response and Antifungal Drug Susceptibility in Cryptococcus neoformans

Kyung Tae Lee, Hyojeong Kwon, Dohyun Lee, Yong Sun Bahn

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A nucleoside diphosphate-linked moiety X (Nudix) hydrolase-like gene, YSA1, has been identified as one of the gromwell plant extract-responsive genes in Cryptococcus neoformans. Ysa1 is known to control intracellular concentrations of ADP-ribose or O-acetyl-ADP-ribose, and has diverse biological functions, including the response to oxidative stress in the ascomycete yeast, Saccharomyces cerevisiae. In this study, we characterized the role of YSA1 in the stress response and adaptation of the basidiomycete yeast, C. neoformans. We constructed three independent deletion mutants for YSA1, and analyzed their mutant phenotypes. We found that ysa1 mutants did not show increased sensitivity to reactive oxygen species-producing oxidative damage agents, such as hydrogen peroxide and menadione, but exhibited increased sensitivity to diamide, which is a thiol-specific oxidant. Ysa1 was dispensable for the response to most environmental stresses, such as genotoxic, osmotic, and endoplasmic reticulum stress. In conclusion, modulation of YSA1 may regulate the cellular response and adaptation of C. neoformans to certain oxidative stresses and contribute to the evolution of antifungal drug resistance.

Original languageEnglish
Pages (from-to)52-58
Number of pages7
JournalMycobiology
Volume42
Issue number1
DOIs
Publication statusPublished - 2014 Mar

Fingerprint

Cryptococcus neoformans
antifungal agents
Diphosphates
nucleosides
Hydrolases
hydrolases
Nucleosides
stress response
Oxidative Stress
oxidative stress
ribose
O-Acetyl-ADP-Ribose
mutants
Fungal Drug Resistance
Lithospermum
Yeasts
Pharmaceutical Preparations
Adenosine Diphosphate Ribose
Diamide
yeasts

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Infectious Diseases

Cite this

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title = "A Nudix Hydrolase Protein, Ysa1, Regulates Oxidative Stress Response and Antifungal Drug Susceptibility in Cryptococcus neoformans",
abstract = "A nucleoside diphosphate-linked moiety X (Nudix) hydrolase-like gene, YSA1, has been identified as one of the gromwell plant extract-responsive genes in Cryptococcus neoformans. Ysa1 is known to control intracellular concentrations of ADP-ribose or O-acetyl-ADP-ribose, and has diverse biological functions, including the response to oxidative stress in the ascomycete yeast, Saccharomyces cerevisiae. In this study, we characterized the role of YSA1 in the stress response and adaptation of the basidiomycete yeast, C. neoformans. We constructed three independent deletion mutants for YSA1, and analyzed their mutant phenotypes. We found that ysa1 mutants did not show increased sensitivity to reactive oxygen species-producing oxidative damage agents, such as hydrogen peroxide and menadione, but exhibited increased sensitivity to diamide, which is a thiol-specific oxidant. Ysa1 was dispensable for the response to most environmental stresses, such as genotoxic, osmotic, and endoplasmic reticulum stress. In conclusion, modulation of YSA1 may regulate the cellular response and adaptation of C. neoformans to certain oxidative stresses and contribute to the evolution of antifungal drug resistance.",
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A Nudix Hydrolase Protein, Ysa1, Regulates Oxidative Stress Response and Antifungal Drug Susceptibility in Cryptococcus neoformans. / Lee, Kyung Tae; Kwon, Hyojeong; Lee, Dohyun; Bahn, Yong Sun.

In: Mycobiology, Vol. 42, No. 1, 03.2014, p. 52-58.

Research output: Contribution to journalArticle

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