A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution

Yong Kyun Sim; Heetae Jung; Su Hyun Kim; Jung Woo Park; Woo Jin Park; Chul Ho Jun

doi:10.1039/c8sc02565g

A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution

Yong Kyun Sim, Heetae Jung, Su Hyun Kim, Jung Woo Park, Woo Jin Park, Chul Ho Jun

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activates the silica surface enough to react with bifunctional linker without destroying activity of biomolecule.

Original language	English
Pages (from-to)	7981-7985
Number of pages	5
Journal	Chemical Science
Volume	9
Issue number	41
DOIs	https://doi.org/10.1039/c8sc02565g
Publication status	Published - 2018

Bibliographical note

Funding Information:
This study was supported nancially by a grant from the National Research Foundation of Korea (Grant 2016-R-1-A2b4009460).

Publisher Copyright:
© 2018 The Royal Society of Chemistry.

All Science Journal Classification (ASJC) codes

Chemistry(all)

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10.1039/c8sc02565g

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abstract = "A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activates the silica surface enough to react with bifunctional linker without destroying activity of biomolecule.",

author = "Sim, {Yong Kyun} and Heetae Jung and Kim, {Su Hyun} and Park, {Jung Woo} and Park, {Woo Jin} and Jun, {Chul Ho}",

note = "Funding Information: This study was supported nancially by a grant from the National Research Foundation of Korea (Grant 2016-R-1-A2b4009460). Publisher Copyright: {\textcopyright} 2018 The Royal Society of Chemistry.",

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language = "English",

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T1 - A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution

AU - Sim, Yong Kyun

AU - Jung, Heetae

AU - Kim, Su Hyun

AU - Park, Jung Woo

AU - Park, Woo Jin

AU - Jun, Chul Ho

N1 - Funding Information: This study was supported nancially by a grant from the National Research Foundation of Korea (Grant 2016-R-1-A2b4009460). Publisher Copyright: © 2018 The Royal Society of Chemistry.

PY - 2018

Y1 - 2018

N2 - A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activates the silica surface enough to react with bifunctional linker without destroying activity of biomolecule.

AB - A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activates the silica surface enough to react with bifunctional linker without destroying activity of biomolecule.

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U2 - 10.1039/c8sc02565g

DO - 10.1039/c8sc02565g

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VL - 9

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JO - Chemical Science

JF - Chemical Science

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ER -

A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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