A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activates the silica surface enough to react with bifunctional linker without destroying activity of biomolecule.
Bibliographical noteFunding Information:
This study was supported nancially by a grant from the National Research Foundation of Korea (Grant 2016-R-1-A2b4009460).
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