A PCR-RFLP Method for the Detection of Activated H-ras Oncogene with a Point Mutation at Codon 12 and 61

Sung Joon Hong, Tack Lee, Young Sug Park, Kyung Ok Lee, Byung Ha Chung, Soo Hyung Lee

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To investigate the incidence of the H-ras gene activation in bladder tumor and the feasibility of using urinary washout samples for screening, a series of 33 human bladder tumors and their preoperatively collected urinary washout samples were screened using a mutant specific PCR-RFLP (polymerase chain-restriction fragment length polymorphism) to detect a point mutation of the H-ras gene. Five tumors were found to harbor H-ras mutations where two tumors had a glycine to valine (G→T) change in codon 12 and three tumors had a glutamine to lysine (C→A) change in codon 61, respectively. Moreover, we could also detect the same point mutations of the H-ras gene in corresponding urine washout samples. The incidence of H-ras mutation in Korean bladder cancer was estimated at approximately 15.2%. In conclusion, a mutant specific PCR-RFLP method for the detection of H-ras gene mutation is useful for screening or postoperative follow-up of bladder tumor due to its simplicity and high specificity even in urinary samples.

Original languageEnglish
Pages (from-to)371-379
Number of pages9
JournalYonsei medical journal
Issue number6
Publication statusPublished - 1996 Jan 1


All Science Journal Classification (ASJC) codes

  • Medicine(all)

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