A pH-sensitive excited state intramolecular proton transfer fluorescent probe for imaging mitochondria and Helicobacter pylori

Kyeong Im Hong, Seong Hyun Park, Seong Min Lee, Injae Shin, Woo Dong Jang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

An hydroxythiophene-conjugated benzothiazole (T2(OH)B) was synthesized as a pH sensitive fluorescent probe. T2(OH)B displayed dual emission behavior due to the excited state intramolecular proton transfer (ESIPT) process, while the methoxy analogs, T2(OMe)B, did not. T2(OH)B showed strong fluorescence enhancement above physiological pHs. The applicability of T2(OH)B as a biological pH indicator was demonstrated by its use for staining mitochondrial compartment and H. pylori. Owing to its strong fluorescence emission with a large stokes shift and its effective response to pH, T2(OH)B serves as a platform for the design of new types of fluorescent probes.

Original languageEnglish
Pages (from-to)148-153
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume286
DOIs
Publication statusPublished - 2019 May 1

Fingerprint

Mitochondria
Proton transfer
mitochondria
Fluorescent Dyes
Excited states
Fluorescence
Imaging techniques
protons
probes
excitation
fluorescence
staining
compartments
platforms
analogs
augmentation
shift
benzothiazole

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

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abstract = "An hydroxythiophene-conjugated benzothiazole (T2(OH)B) was synthesized as a pH sensitive fluorescent probe. T2(OH)B displayed dual emission behavior due to the excited state intramolecular proton transfer (ESIPT) process, while the methoxy analogs, T2(OMe)B, did not. T2(OH)B showed strong fluorescence enhancement above physiological pHs. The applicability of T2(OH)B as a biological pH indicator was demonstrated by its use for staining mitochondrial compartment and H. pylori. Owing to its strong fluorescence emission with a large stokes shift and its effective response to pH, T2(OH)B serves as a platform for the design of new types of fluorescent probes.",
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A pH-sensitive excited state intramolecular proton transfer fluorescent probe for imaging mitochondria and Helicobacter pylori. / Hong, Kyeong Im; Park, Seong Hyun; Lee, Seong Min; Shin, Injae; Jang, Woo Dong.

In: Sensors and Actuators, B: Chemical, Vol. 286, 01.05.2019, p. 148-153.

Research output: Contribution to journalArticle

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T1 - A pH-sensitive excited state intramolecular proton transfer fluorescent probe for imaging mitochondria and Helicobacter pylori

AU - Hong, Kyeong Im

AU - Park, Seong Hyun

AU - Lee, Seong Min

AU - Shin, Injae

AU - Jang, Woo Dong

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