A photothermal biosensor for detection of C-reactive protein in human saliva

Seung Hyeon Lee, Seoyeon Choi, Kirok Kwon, Nam Ho Bae, Bong Seop Kwak, Won Chang Cho, Seok Jae Lee, Hyo Il Jung

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In this study, our objective was to develop a photothermal biosensor (PTB) for measurement of C-reactive protein (CRP) concentrations in human saliva. The resulting PTB that we developed is rapid, simple, and minimally invasive, and can be used to measure the concentration of CRP in human saliva (7-μL sample volume). The CRP (0.1–100 ng/mL) is captured by the sandwich immunoassay on the sensor, and gold nanoparticles (GNPs) conjugated with antibodies cause a photothermal effect when irradiated with a laser (90 s, wavelength = 532 nm). The temperature changes caused by this effect correlate with the concentrations of CRP. Therefore, the sensor shows a higher correlation with the concentrations of CRP than commercial detection kits. Its detection limit is 0.1 ng/mL CRP.

Original languageEnglish
Pages (from-to)471-476
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume246
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

saliva
bioinstrumentation
Biosensors
C-Reactive Protein
proteins
Proteins
immunoassay
kits
sensors
Sensors
antibodies
Antibodies
Gold
gold
Nanoparticles
Wavelength
nanoparticles
Lasers
causes
wavelengths

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Lee, Seung Hyeon ; Choi, Seoyeon ; Kwon, Kirok ; Bae, Nam Ho ; Kwak, Bong Seop ; Cho, Won Chang ; Lee, Seok Jae ; Jung, Hyo Il. / A photothermal biosensor for detection of C-reactive protein in human saliva. In: Sensors and Actuators, B: Chemical. 2017 ; Vol. 246. pp. 471-476.
@article{8060a7a6fee24a59b018d01a85f04d7c,
title = "A photothermal biosensor for detection of C-reactive protein in human saliva",
abstract = "In this study, our objective was to develop a photothermal biosensor (PTB) for measurement of C-reactive protein (CRP) concentrations in human saliva. The resulting PTB that we developed is rapid, simple, and minimally invasive, and can be used to measure the concentration of CRP in human saliva (7-μL sample volume). The CRP (0.1–100 ng/mL) is captured by the sandwich immunoassay on the sensor, and gold nanoparticles (GNPs) conjugated with antibodies cause a photothermal effect when irradiated with a laser (90 s, wavelength = 532 nm). The temperature changes caused by this effect correlate with the concentrations of CRP. Therefore, the sensor shows a higher correlation with the concentrations of CRP than commercial detection kits. Its detection limit is 0.1 ng/mL CRP.",
author = "Lee, {Seung Hyeon} and Seoyeon Choi and Kirok Kwon and Bae, {Nam Ho} and Kwak, {Bong Seop} and Cho, {Won Chang} and Lee, {Seok Jae} and Jung, {Hyo Il}",
year = "2017",
month = "1",
day = "1",
doi = "10.1016/j.snb.2017.01.188",
language = "English",
volume = "246",
pages = "471--476",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",

}

A photothermal biosensor for detection of C-reactive protein in human saliva. / Lee, Seung Hyeon; Choi, Seoyeon; Kwon, Kirok; Bae, Nam Ho; Kwak, Bong Seop; Cho, Won Chang; Lee, Seok Jae; Jung, Hyo Il.

In: Sensors and Actuators, B: Chemical, Vol. 246, 01.01.2017, p. 471-476.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A photothermal biosensor for detection of C-reactive protein in human saliva

AU - Lee, Seung Hyeon

AU - Choi, Seoyeon

AU - Kwon, Kirok

AU - Bae, Nam Ho

AU - Kwak, Bong Seop

AU - Cho, Won Chang

AU - Lee, Seok Jae

AU - Jung, Hyo Il

PY - 2017/1/1

Y1 - 2017/1/1

N2 - In this study, our objective was to develop a photothermal biosensor (PTB) for measurement of C-reactive protein (CRP) concentrations in human saliva. The resulting PTB that we developed is rapid, simple, and minimally invasive, and can be used to measure the concentration of CRP in human saliva (7-μL sample volume). The CRP (0.1–100 ng/mL) is captured by the sandwich immunoassay on the sensor, and gold nanoparticles (GNPs) conjugated with antibodies cause a photothermal effect when irradiated with a laser (90 s, wavelength = 532 nm). The temperature changes caused by this effect correlate with the concentrations of CRP. Therefore, the sensor shows a higher correlation with the concentrations of CRP than commercial detection kits. Its detection limit is 0.1 ng/mL CRP.

AB - In this study, our objective was to develop a photothermal biosensor (PTB) for measurement of C-reactive protein (CRP) concentrations in human saliva. The resulting PTB that we developed is rapid, simple, and minimally invasive, and can be used to measure the concentration of CRP in human saliva (7-μL sample volume). The CRP (0.1–100 ng/mL) is captured by the sandwich immunoassay on the sensor, and gold nanoparticles (GNPs) conjugated with antibodies cause a photothermal effect when irradiated with a laser (90 s, wavelength = 532 nm). The temperature changes caused by this effect correlate with the concentrations of CRP. Therefore, the sensor shows a higher correlation with the concentrations of CRP than commercial detection kits. Its detection limit is 0.1 ng/mL CRP.

UR - http://www.scopus.com/inward/record.url?scp=85013864948&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85013864948&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2017.01.188

DO - 10.1016/j.snb.2017.01.188

M3 - Article

AN - SCOPUS:85013864948

VL - 246

SP - 471

EP - 476

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

ER -