A polyethylene glycol-based hydrogel as macroporous scaffold for tumorsphere formation of glioblastoma multiforme

Yoonjee Oh, Junghwa Cha, Seok-Gu Kang, Pilnam Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We report a formation of macroporous scaffold which is based on polyethylene glycol (PEG)-based alginate (ALG) interpenetrating polymer network (IPN) hydrogel. Using this scaffold, we assess the tumorsphere (TS)-forming ability of glioma cancer stem cells (gCSCs), which subpopulation has been highlighted as a main cause of therapeutic resistance due to self-renewal and potential of differentiation properties. Although there have been numerous methods to study the TSs, however, there is no plausible method to evaluate the formation of single gCSC cell-derived TSs, due to fusion-induced cell aggregation. To provide reliable assessment, the PEGDA hydrogel interpenetrated with ALG was fabricated as macroporous scaffold for TS formation of patient-derived gCSCs. With UV-ionic dual crosslinking process, the pore size of PEGDA-ALG hydrogel is magnified enough to be applied as a macroporous scaffold, providing increased internal voids for TS growth and expansion. As a result, within macroporous scaffold, the multiple number of single gCSC-derived TSs was successfully formed inside the structural voids.

Original languageEnglish
Pages (from-to)10-15
Number of pages6
JournalJournal of Industrial and Engineering Chemistry
Volume39
DOIs
Publication statusPublished - 2016 Jul 25

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Hydrogel
Hydrogels
Scaffolds
Polyethylene glycols
Stem cells
Alginate
Interpenetrating polymer networks
Crosslinking
Pore size
Fusion reactions
Agglomeration
alginic acid
poly(ethylene glycol)diacrylate

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)

Cite this

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abstract = "We report a formation of macroporous scaffold which is based on polyethylene glycol (PEG)-based alginate (ALG) interpenetrating polymer network (IPN) hydrogel. Using this scaffold, we assess the tumorsphere (TS)-forming ability of glioma cancer stem cells (gCSCs), which subpopulation has been highlighted as a main cause of therapeutic resistance due to self-renewal and potential of differentiation properties. Although there have been numerous methods to study the TSs, however, there is no plausible method to evaluate the formation of single gCSC cell-derived TSs, due to fusion-induced cell aggregation. To provide reliable assessment, the PEGDA hydrogel interpenetrated with ALG was fabricated as macroporous scaffold for TS formation of patient-derived gCSCs. With UV-ionic dual crosslinking process, the pore size of PEGDA-ALG hydrogel is magnified enough to be applied as a macroporous scaffold, providing increased internal voids for TS growth and expansion. As a result, within macroporous scaffold, the multiple number of single gCSC-derived TSs was successfully formed inside the structural voids.",
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A polyethylene glycol-based hydrogel as macroporous scaffold for tumorsphere formation of glioblastoma multiforme. / Oh, Yoonjee; Cha, Junghwa; Kang, Seok-Gu; Kim, Pilnam.

In: Journal of Industrial and Engineering Chemistry, Vol. 39, 25.07.2016, p. 10-15.

Research output: Contribution to journalArticle

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