We report a formation of macroporous scaffold which is based on polyethylene glycol (PEG)-based alginate (ALG) interpenetrating polymer network (IPN) hydrogel. Using this scaffold, we assess the tumorsphere (TS)-forming ability of glioma cancer stem cells (gCSCs), which subpopulation has been highlighted as a main cause of therapeutic resistance due to self-renewal and potential of differentiation properties. Although there have been numerous methods to study the TSs, however, there is no plausible method to evaluate the formation of single gCSC cell-derived TSs, due to fusion-induced cell aggregation. To provide reliable assessment, the PEGDA hydrogel interpenetrated with ALG was fabricated as macroporous scaffold for TS formation of patient-derived gCSCs. With UV-ionic dual crosslinking process, the pore size of PEGDA-ALG hydrogel is magnified enough to be applied as a macroporous scaffold, providing increased internal voids for TS growth and expansion. As a result, within macroporous scaffold, the multiple number of single gCSC-derived TSs was successfully formed inside the structural voids.
Bibliographical noteFunding Information:
We greatly appreciate to Dr. Frederick F. Lang (Department of Neurosurgery, The University of Texas, MD Anderson Cancer Center, Texas, USA) for providing patient-derived, GBM tumorsphere-forming GSC11 cells. This research was supported from the National Research Foundation of Korea (NRF) (grant number: NRF-2014R1A1A1004985 , NRF-2015M3A9B3028685 and NRF-2015H1A2A1030560 ) and by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C00420200 , HI14C1324 ).
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)