The plant hormone ethylene has been shown to play an important role in root hair development in Arabidopsis. With the aid of proteomic analysis, we identified three distinct glutathione S-transferase (GST) isoforms, AtGSTF2, AtGSTF8, and AtGSTU19, expressed early in root epidermal establishment in Arabidopsis seedlings. The AtGSTF2 protein was specifically up-regulated by ethylene. A subsequent RNA expression study revealed that the AtGSTF2 gene was highly sensitive to ethylene, whereas the transcripts for AtGSTF8 and AtGSTU19 were constitutively present in new root tissue of 4-day-old seedlings. The steady-state level of AtGSTF2 mRNA was greatly reduced in the roots of ethylene-insensitive mutants, while mutation at the CTR1 locus, which confers an ectopic root hair phenotype, resulted in a markedly elevated level of AtGSTF2 transcript in young root tissue. Although the physiological function of ethylene-induced AtGSTF2 is not yet clear, there are several possibilities for its role during early root development.
|Number of pages||9|
|Journal||Biochimica et Biophysica Acta - Gene Structure and Expression|
|Publication status||Published - 2004 Feb 20|
Bibliographical noteFunding Information:
This work was supported by grants from Plant Metabolism Research Center at Kyung Hee University (Science Research Center Program from Korea Science and Engineering Foundation), Plant Diversity Research Center (21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean government, project number PF0330404-00), and KRIBB (joint research program) to W.T.K., and from KOSEF (Protein Network Research Center at Yonsei University) to Y.Y.B.
All Science Journal Classification (ASJC) codes
- Structural Biology