A proteomic analysis identifies glutathione S-transferase isoforms whose abundance is differentially regulated by ethylene during the formation of early root epidermis in Arabidopsis seedlings

Hyung Gon Mang, Eun Ok Kang, Jung Hye Shim, So Yeon Kim, Ky Young Park, Yu Sam Kim, Young Yil Bahk, Woo Taek Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The plant hormone ethylene has been shown to play an important role in root hair development in Arabidopsis. With the aid of proteomic analysis, we identified three distinct glutathione S-transferase (GST) isoforms, AtGSTF2, AtGSTF8, and AtGSTU19, expressed early in root epidermal establishment in Arabidopsis seedlings. The AtGSTF2 protein was specifically up-regulated by ethylene. A subsequent RNA expression study revealed that the AtGSTF2 gene was highly sensitive to ethylene, whereas the transcripts for AtGSTF8 and AtGSTU19 were constitutively present in new root tissue of 4-day-old seedlings. The steady-state level of AtGSTF2 mRNA was greatly reduced in the roots of ethylene-insensitive mutants, while mutation at the CTR1 locus, which confers an ectopic root hair phenotype, resulted in a markedly elevated level of AtGSTF2 transcript in young root tissue. Although the physiological function of ethylene-induced AtGSTF2 is not yet clear, there are several possibilities for its role during early root development.

Original languageEnglish
Pages (from-to)231-239
Number of pages9
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1676
Issue number3
DOIs
Publication statusPublished - 2004 Feb 20

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Glutathione Transferase
Seedlings
Arabidopsis
Epidermis
Proteomics
Protein Isoforms
Tissue
Plant Growth Regulators
Genes
ethylene
RNA
Phenotype
Messenger RNA
Mutation
Proteins

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

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title = "A proteomic analysis identifies glutathione S-transferase isoforms whose abundance is differentially regulated by ethylene during the formation of early root epidermis in Arabidopsis seedlings",
abstract = "The plant hormone ethylene has been shown to play an important role in root hair development in Arabidopsis. With the aid of proteomic analysis, we identified three distinct glutathione S-transferase (GST) isoforms, AtGSTF2, AtGSTF8, and AtGSTU19, expressed early in root epidermal establishment in Arabidopsis seedlings. The AtGSTF2 protein was specifically up-regulated by ethylene. A subsequent RNA expression study revealed that the AtGSTF2 gene was highly sensitive to ethylene, whereas the transcripts for AtGSTF8 and AtGSTU19 were constitutively present in new root tissue of 4-day-old seedlings. The steady-state level of AtGSTF2 mRNA was greatly reduced in the roots of ethylene-insensitive mutants, while mutation at the CTR1 locus, which confers an ectopic root hair phenotype, resulted in a markedly elevated level of AtGSTF2 transcript in young root tissue. Although the physiological function of ethylene-induced AtGSTF2 is not yet clear, there are several possibilities for its role during early root development.",
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A proteomic analysis identifies glutathione S-transferase isoforms whose abundance is differentially regulated by ethylene during the formation of early root epidermis in Arabidopsis seedlings. / Mang, Hyung Gon; Kang, Eun Ok; Shim, Jung Hye; Kim, So Yeon; Park, Ky Young; Kim, Yu Sam; Bahk, Young Yil; Kim, Woo Taek.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1676, No. 3, 20.02.2004, p. 231-239.

Research output: Contribution to journalArticle

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AU - Kang, Eun Ok

AU - Shim, Jung Hye

AU - Kim, So Yeon

AU - Park, Ky Young

AU - Kim, Yu Sam

AU - Bahk, Young Yil

AU - Kim, Woo Taek

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