A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy

Kyujung Kim, Donghyun Kim, Eun Jin Cho, Yong Min Huh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

For investigatoin of intracellular protein interactions, quantum dots are widely used for fluorescent live cell imaging such as total internal reflection fluorescence (TIRF) microscopy and confocal microscopy. In this paper, we performed a quantitative analysis based on fluorescent intensity. For the measurement, A431 cell lines are imaged live with quantum dots using TIRF microscopy. The distribution of quantum dots is affected by a TATHA2 peptide sequence in live cells. This paper also presents the location change of quantum dots due to a nuclear localization signal in A431 cell lines. Confocal microscopy was used to confirm the relation with fluorescent intensity and quantum dot concentration in live cells.

Original languageEnglish
Title of host publicationGenetically Engineered and Optical Probes for Biomedical Applications IV
DOIs
Publication statusPublished - 2007
EventGenetically Engineered and Optical Probes for Biomedical Applications IV - San Jose, CA, United States
Duration: 2007 Jan 232007 Jan 24

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6449
ISSN (Print)1605-7422

Other

OtherGenetically Engineered and Optical Probes for Biomedical Applications IV
CountryUnited States
CitySan Jose, CA
Period07/1/2307/1/24

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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    Kim, K., Kim, D., Cho, E. J., & Huh, Y. M. (2007). A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy. In Genetically Engineered and Optical Probes for Biomedical Applications IV [64490K] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6449). https://doi.org/10.1117/12.699874