A rapid amplification/detection assay for analysis of Mycobacterium tuberculosis using an isothermal and silicon bio-photonic sensor complex

Yong Shin, Agampodi Promoda Perera, Wen Ying Tang, Dong Liang Fu, Qing Liu, Jack Kee Sheng, Zhonghua Gu, Tae Yoon Lee, Timothy Barkham, Mi Kyoung Park

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Global tuberculosis (TB) control is hampered by cost and slow or insensitive diagnostic methods to be used for TB diagnosis in clinic. Thus, TB still remains a major global health problem. The failure to rapidly and accurately diagnose of TB has posed significant challenges with consequent secondary resistance and ongoing transmission. We developed a rapid Mycobacterium tuberculosis (MTB) amplification/detection method, called MTB isothermal solid-phase amplification/detection (MTB-ISAD), that couples isothermal solid-phase amplification and a silicon biophotonics-based detection sensor to allow the simultaneous amplification and detection of MTB in a label-free and real-time manner. We validated the clinical utility of the MTB-ISAD assay by detecting MTB nucleic acid in sputum samples from 42 patients. We showed the ability of the MTB-ISAD assay to detect MTB in 42 clinical specimens, confirming that the MTB-ISAD assay is fast (<20. min), highly sensitive, accurate (>90%, 38/42), and cost-effective because it is a label-free method and does not involve thermal cycling. The MTB-ISAD assay has improved time-efficiency, affordability, and sensitivity compared with many existing methods. Therefore, it is potentially adaptable for better diagnosis across various clinical applications.

Original languageEnglish
Pages (from-to)390-396
Number of pages7
JournalBiosensors and Bioelectronics
Volume68
DOIs
Publication statusPublished - 2015 Jun 5

Bibliographical note

Funding Information:
This work was supported by the Agency for Science, Technology and Research (A ⁎ STAR) Joint Council Office (JCO) Development Program Grant ( 1234e00018 ), Singapore

Publisher Copyright:
© 2015 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Fingerprint

Dive into the research topics of 'A rapid amplification/detection assay for analysis of Mycobacterium tuberculosis using an isothermal and silicon bio-photonic sensor complex'. Together they form a unique fingerprint.

Cite this