Abstract
Global tuberculosis (TB) control is hampered by cost and slow or insensitive diagnostic methods to be used for TB diagnosis in clinic. Thus, TB still remains a major global health problem. The failure to rapidly and accurately diagnose of TB has posed significant challenges with consequent secondary resistance and ongoing transmission. We developed a rapid Mycobacterium tuberculosis (MTB) amplification/detection method, called MTB isothermal solid-phase amplification/detection (MTB-ISAD), that couples isothermal solid-phase amplification and a silicon biophotonics-based detection sensor to allow the simultaneous amplification and detection of MTB in a label-free and real-time manner. We validated the clinical utility of the MTB-ISAD assay by detecting MTB nucleic acid in sputum samples from 42 patients. We showed the ability of the MTB-ISAD assay to detect MTB in 42 clinical specimens, confirming that the MTB-ISAD assay is fast (<20. min), highly sensitive, accurate (>90%, 38/42), and cost-effective because it is a label-free method and does not involve thermal cycling. The MTB-ISAD assay has improved time-efficiency, affordability, and sensitivity compared with many existing methods. Therefore, it is potentially adaptable for better diagnosis across various clinical applications.
Original language | English |
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Pages (from-to) | 390-396 |
Number of pages | 7 |
Journal | Biosensors and Bioelectronics |
Volume | 68 |
DOIs | |
Publication status | Published - 2015 Jun 5 |
Bibliographical note
Funding Information:This work was supported by the Agency for Science, Technology and Research (A ⁎ STAR) Joint Council Office (JCO) Development Program Grant ( 1234e00018 ), Singapore
Publisher Copyright:
© 2015 Elsevier B.V.
All Science Journal Classification (ASJC) codes
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry