A rapid and chemical-free hemoglobin assay with photothermal angular light scattering

Uihan Kim, Jaewoo Song, Suho Ryu, Soocheol Kim, Chulmin Joo

Research output: Contribution to journalArticle

Abstract

Photo-thermal angular light scattering (PT-AS) is a novel optical method for measuring the hemoglobin concentration ([Hb]) of blood samples. On the basis of the intrinsic photothermal response of hemoglobin molecules, the sensor enables high-sensitivity, chemical-free measurement of [Hb]. [Hb] detection capability with a limit of 0.12 g/dl over the range of 0.35 - 17.9 g/dl has been demonstrated previously. The method can be readily implemented using inexpensive consumer electronic devices such as a laser pointer and a webcam. The use of a micro-capillary tube as a blood container also enables the hemoglobin assay with a nanoliter-scale blood volume and a low operating cost. Here, detailed instructions for the PT-AS optical setup and signal processing procedures are presented. Experimental protocols and representative results for blood samples in anemic conditions ([Hb] = 5.3, 7.5, and 9.9 g/dl) are also provided, and the measurements are compared with those from a hematology analyzer. Its simplicity in implementation and operation should enable its wide adoption in clinical laboratories and resource-limited settings.

Original languageEnglish
Article numbere55006
JournalJournal of Visualized Experiments
Volume2016
Issue number118
DOIs
Publication statusPublished - 2016 Dec 7

Fingerprint

Hemoglobin
Light scattering
Assays
Hemoglobins
Blood
Light
Hot Temperature
Clinical laboratories
Hematology
Blood Volume
Capillary tubes
Consumer electronics
Lasers
Operating costs
Containers
Costs and Cost Analysis
Equipment and Supplies
Signal processing
Molecules
Sensors

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Kim, Uihan ; Song, Jaewoo ; Ryu, Suho ; Kim, Soocheol ; Joo, Chulmin. / A rapid and chemical-free hemoglobin assay with photothermal angular light scattering. In: Journal of Visualized Experiments. 2016 ; Vol. 2016, No. 118.
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A rapid and chemical-free hemoglobin assay with photothermal angular light scattering. / Kim, Uihan; Song, Jaewoo; Ryu, Suho; Kim, Soocheol; Joo, Chulmin.

In: Journal of Visualized Experiments, Vol. 2016, No. 118, e55006, 07.12.2016.

Research output: Contribution to journalArticle

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