A regulatory role for phenol oxidase during decomposition in peatlands

C. Freeman, N. J. Ostle, N. Fenner, Hojeong Kang

Research output: Contribution to journalArticle

218 Citations (Scopus)

Abstract

Unique peatland properties, such as their ability to preserve intact ancient human remains (bog bodies) and to store globally significant quantities of atmospheric CO 2, can be attributed to their low rates of enzymic decomposition. Peatland soils are normally devoid of molecular oxygen in all, but the uppermost layer, and thus enzymes such as phenol oxidase, which require molecular oxygen for their activity, are rarely active. Interestingly, even the activities of enzymes such as hydrolases that have no oxygen requirement, are also extremely limited in peatlands. Here, we show that those low hydrolase activities can be indirectly attributed to oxygen constraints on phenol oxidase. On addition of oxygen, phenol oxidase activity increased 7-fold, P<0.05, a response that allowed phenolic depletion in the peatland soil. Phenolic materials are highly inhibitory to enzymes and their lower abundance allowed higher hydrolase activities (β-glucosidase 26%, P<0.05, phosphatase 18%, P<0.05, sulphatase 47%, P<0.01, xylosidase 16%, P<0.05 and chitinase 22%, P<0.05). Thus, oxygen constraints upon phenol oxidase activity promote conditions that inhibit decomposition. This mechanism has important implications for preservation of archaeological organic materials, sequestration of atmospheric CO 2 and potentially in the preservation of food and treatment of water pollution.

Original languageEnglish
Pages (from-to)1663-1667
Number of pages5
JournalSoil Biology and Biochemistry
Volume36
Issue number10
DOIs
Publication statusPublished - 2004 Oct 1

Fingerprint

Monophenol Monooxygenase
monophenol monooxygenase
peatlands
peatland
phenol
decomposition
Oxygen
oxygen
hydrolases
degradation
Hydrolases
Carbon Monoxide
enzyme
oxygen requirement
Enzymes
Soil
Xylosidases
arylsulfatase
glucosidases
Food Preservation

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Soil Science

Cite this

Freeman, C. ; Ostle, N. J. ; Fenner, N. ; Kang, Hojeong. / A regulatory role for phenol oxidase during decomposition in peatlands. In: Soil Biology and Biochemistry. 2004 ; Vol. 36, No. 10. pp. 1663-1667.
@article{4183e788f35941d199cc0adfd6e7809a,
title = "A regulatory role for phenol oxidase during decomposition in peatlands",
abstract = "Unique peatland properties, such as their ability to preserve intact ancient human remains (bog bodies) and to store globally significant quantities of atmospheric CO 2, can be attributed to their low rates of enzymic decomposition. Peatland soils are normally devoid of molecular oxygen in all, but the uppermost layer, and thus enzymes such as phenol oxidase, which require molecular oxygen for their activity, are rarely active. Interestingly, even the activities of enzymes such as hydrolases that have no oxygen requirement, are also extremely limited in peatlands. Here, we show that those low hydrolase activities can be indirectly attributed to oxygen constraints on phenol oxidase. On addition of oxygen, phenol oxidase activity increased 7-fold, P<0.05, a response that allowed phenolic depletion in the peatland soil. Phenolic materials are highly inhibitory to enzymes and their lower abundance allowed higher hydrolase activities (β-glucosidase 26{\%}, P<0.05, phosphatase 18{\%}, P<0.05, sulphatase 47{\%}, P<0.01, xylosidase 16{\%}, P<0.05 and chitinase 22{\%}, P<0.05). Thus, oxygen constraints upon phenol oxidase activity promote conditions that inhibit decomposition. This mechanism has important implications for preservation of archaeological organic materials, sequestration of atmospheric CO 2 and potentially in the preservation of food and treatment of water pollution.",
author = "C. Freeman and Ostle, {N. J.} and N. Fenner and Hojeong Kang",
year = "2004",
month = "10",
day = "1",
doi = "10.1016/j.soilbio.2004.07.012",
language = "English",
volume = "36",
pages = "1663--1667",
journal = "Soil Biology and Biochemistry",
issn = "0038-0717",
publisher = "Elsevier Limited",
number = "10",

}

A regulatory role for phenol oxidase during decomposition in peatlands. / Freeman, C.; Ostle, N. J.; Fenner, N.; Kang, Hojeong.

In: Soil Biology and Biochemistry, Vol. 36, No. 10, 01.10.2004, p. 1663-1667.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A regulatory role for phenol oxidase during decomposition in peatlands

AU - Freeman, C.

AU - Ostle, N. J.

AU - Fenner, N.

AU - Kang, Hojeong

PY - 2004/10/1

Y1 - 2004/10/1

N2 - Unique peatland properties, such as their ability to preserve intact ancient human remains (bog bodies) and to store globally significant quantities of atmospheric CO 2, can be attributed to their low rates of enzymic decomposition. Peatland soils are normally devoid of molecular oxygen in all, but the uppermost layer, and thus enzymes such as phenol oxidase, which require molecular oxygen for their activity, are rarely active. Interestingly, even the activities of enzymes such as hydrolases that have no oxygen requirement, are also extremely limited in peatlands. Here, we show that those low hydrolase activities can be indirectly attributed to oxygen constraints on phenol oxidase. On addition of oxygen, phenol oxidase activity increased 7-fold, P<0.05, a response that allowed phenolic depletion in the peatland soil. Phenolic materials are highly inhibitory to enzymes and their lower abundance allowed higher hydrolase activities (β-glucosidase 26%, P<0.05, phosphatase 18%, P<0.05, sulphatase 47%, P<0.01, xylosidase 16%, P<0.05 and chitinase 22%, P<0.05). Thus, oxygen constraints upon phenol oxidase activity promote conditions that inhibit decomposition. This mechanism has important implications for preservation of archaeological organic materials, sequestration of atmospheric CO 2 and potentially in the preservation of food and treatment of water pollution.

AB - Unique peatland properties, such as their ability to preserve intact ancient human remains (bog bodies) and to store globally significant quantities of atmospheric CO 2, can be attributed to their low rates of enzymic decomposition. Peatland soils are normally devoid of molecular oxygen in all, but the uppermost layer, and thus enzymes such as phenol oxidase, which require molecular oxygen for their activity, are rarely active. Interestingly, even the activities of enzymes such as hydrolases that have no oxygen requirement, are also extremely limited in peatlands. Here, we show that those low hydrolase activities can be indirectly attributed to oxygen constraints on phenol oxidase. On addition of oxygen, phenol oxidase activity increased 7-fold, P<0.05, a response that allowed phenolic depletion in the peatland soil. Phenolic materials are highly inhibitory to enzymes and their lower abundance allowed higher hydrolase activities (β-glucosidase 26%, P<0.05, phosphatase 18%, P<0.05, sulphatase 47%, P<0.01, xylosidase 16%, P<0.05 and chitinase 22%, P<0.05). Thus, oxygen constraints upon phenol oxidase activity promote conditions that inhibit decomposition. This mechanism has important implications for preservation of archaeological organic materials, sequestration of atmospheric CO 2 and potentially in the preservation of food and treatment of water pollution.

UR - http://www.scopus.com/inward/record.url?scp=5344230062&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=5344230062&partnerID=8YFLogxK

U2 - 10.1016/j.soilbio.2004.07.012

DO - 10.1016/j.soilbio.2004.07.012

M3 - Article

VL - 36

SP - 1663

EP - 1667

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

IS - 10

ER -