A report of cat scratch disease in Korea confirmed by PCR amplification of the 16S-23S rRNA intergenic region of Bartonella henselae

Borum Suh, Jin Kyoung Chun, Dongeun Yong, Yang Soon Lee, Seok Hoon Jeong, Woo Ick Yang, Dong Soo Kim

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

We report a case of cat scratch disease in an 8-yr-old girl who presented with fever and enlargement of both axillary lymph nodes. Both aerobic and anaerobic cultures of the lymph node aspirate were negative for microbial growth. Gram staining and Warthin-Starry silver staining did not reveal any organism. Purified DNA from the PCR-amplicon of the 16S-23S rRNA intergenic region was sequenced and showed 99.7% identity with the corresponding sequence of Bartonella henselae strain Houston-1. Our findings suggest that the internal transcribed spacer is a reliable region for PCR identification of Bartonella species. In patients with lymphadenitis, a history of contact with cats or dogs necessitates the use of diagnostic approaches that employ not only the conventional staining and culture but also molecular methods to detect B. henselae.

Original languageEnglish
Pages (from-to)34-37
Number of pages4
JournalKorean Journal of Laboratory Medicine
Volume30
Issue number1
DOIs
Publication statusPublished - 2010 Jul 28

Fingerprint

Bartonella henselae
Cat-Scratch Disease
Intergenic DNA
Korea
Silver
Amplification
Lymph Nodes
Bartonella
Staining and Labeling
Polymerase Chain Reaction
Lymphadenitis
Silver Staining
DNA
Cats
Fever
Dogs
Growth

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

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title = "A report of cat scratch disease in Korea confirmed by PCR amplification of the 16S-23S rRNA intergenic region of Bartonella henselae",
abstract = "We report a case of cat scratch disease in an 8-yr-old girl who presented with fever and enlargement of both axillary lymph nodes. Both aerobic and anaerobic cultures of the lymph node aspirate were negative for microbial growth. Gram staining and Warthin-Starry silver staining did not reveal any organism. Purified DNA from the PCR-amplicon of the 16S-23S rRNA intergenic region was sequenced and showed 99.7{\%} identity with the corresponding sequence of Bartonella henselae strain Houston-1. Our findings suggest that the internal transcribed spacer is a reliable region for PCR identification of Bartonella species. In patients with lymphadenitis, a history of contact with cats or dogs necessitates the use of diagnostic approaches that employ not only the conventional staining and culture but also molecular methods to detect B. henselae.",
author = "Borum Suh and Chun, {Jin Kyoung} and Dongeun Yong and Lee, {Yang Soon} and Jeong, {Seok Hoon} and Yang, {Woo Ick} and Kim, {Dong Soo}",
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A report of cat scratch disease in Korea confirmed by PCR amplification of the 16S-23S rRNA intergenic region of Bartonella henselae. / Suh, Borum; Chun, Jin Kyoung; Yong, Dongeun; Lee, Yang Soon; Jeong, Seok Hoon; Yang, Woo Ick; Kim, Dong Soo.

In: Korean Journal of Laboratory Medicine, Vol. 30, No. 1, 28.07.2010, p. 34-37.

Research output: Contribution to journalReview article

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T1 - A report of cat scratch disease in Korea confirmed by PCR amplification of the 16S-23S rRNA intergenic region of Bartonella henselae

AU - Suh, Borum

AU - Chun, Jin Kyoung

AU - Yong, Dongeun

AU - Lee, Yang Soon

AU - Jeong, Seok Hoon

AU - Yang, Woo Ick

AU - Kim, Dong Soo

PY - 2010/7/28

Y1 - 2010/7/28

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AB - We report a case of cat scratch disease in an 8-yr-old girl who presented with fever and enlargement of both axillary lymph nodes. Both aerobic and anaerobic cultures of the lymph node aspirate were negative for microbial growth. Gram staining and Warthin-Starry silver staining did not reveal any organism. Purified DNA from the PCR-amplicon of the 16S-23S rRNA intergenic region was sequenced and showed 99.7% identity with the corresponding sequence of Bartonella henselae strain Houston-1. Our findings suggest that the internal transcribed spacer is a reliable region for PCR identification of Bartonella species. In patients with lymphadenitis, a history of contact with cats or dogs necessitates the use of diagnostic approaches that employ not only the conventional staining and culture but also molecular methods to detect B. henselae.

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