A role for protein disulfide isomerase in the early folding and assembly of MHC class I molecules.

Kwonyoon Kang, Boyoun Park, Changhoon Oh, Kwangmin Cho, Kwangseog Ahn

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Proper folding and assembly of major histocompatibility complex (MHC) class I complexes are essential for optimal peptide loading and subsequent antigen presentation. MHC class I folding involves the coordinated formation of multiple disulfide bonds within MHC class I molecules. However, the regulation of disulfide bond formation during the early process of MHC class I folding is uncharacterized. Here, we show that protein disulfide isomerase (PDI) catalyzes the disulfide bond formation of MHC class I molecules and thereby facilitates the assembly of MHC class I heavy chain with beta(2)-microglobulin (beta(2)m). Depletion of PDI but not ERp57 by RNAi interfered with the disulfide bond formation in the MHC class I molecules. In the absence of PDI, the association of free class I heavy chain with calnexin increased, whereas the assembly of MHC class I heavy chain-beta(2)m heterodimers was delayed. These observations suggest that PDI-catalyzed disulfide bond formation of MHC class I molecules is an event downstream of the interaction of class I molecules with calnexin and upstream of their interaction with beta(2)m. Thus, our data establish a critical function for PDI in the early assembly of MHC class I molecules.

Original languageEnglish
Pages (from-to)2553-2561
Number of pages9
JournalAntioxidants & redox signaling
Volume11
Issue number10
DOIs
Publication statusPublished - 2009 Jan 1

Fingerprint

Protein Disulfide-Isomerases
Major Histocompatibility Complex
Disulfides
beta 2-Microglobulin
Molecules
Calnexin
Free Association
Antigen Presentation
Antigens
Peptides
RNA Interference

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Kang, Kwonyoon ; Park, Boyoun ; Oh, Changhoon ; Cho, Kwangmin ; Ahn, Kwangseog. / A role for protein disulfide isomerase in the early folding and assembly of MHC class I molecules. In: Antioxidants & redox signaling. 2009 ; Vol. 11, No. 10. pp. 2553-2561.
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A role for protein disulfide isomerase in the early folding and assembly of MHC class I molecules. / Kang, Kwonyoon; Park, Boyoun; Oh, Changhoon; Cho, Kwangmin; Ahn, Kwangseog.

In: Antioxidants & redox signaling, Vol. 11, No. 10, 01.01.2009, p. 2553-2561.

Research output: Contribution to journalArticle

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