A role of hexokinases in plant resistance to oxidative stress and pathogen infection

Sujon Sarowar, Jae Yong Lee, Eu Ree Ahn, Hyun Sook Pai

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Previously, we reported that mitochondria-associated hexokinases are active in controlling programmed cell death in plants (Plant Cell 18, 2341-2355). Here, we investigated their role under abiotic- and biotic-stress conditions. Expression of NbHxk1, a Nicotiana benthamiana hexokinase gene, was stimulated by treatment with salicylic acid or methyl viologen (MV), and was also up-regulated by pathogen infection. In response to MV-induced oxidative stress, NbHxk1-silenced plants exhibited increased susceptibility, while the HXK1- and HXK2-overexpressing Arabidopsis plants had enhanced tolerance. Moreover, those overexpressing plants showed greater resistance to the necrotrophic fungal pathogen Alternaria brassicicola. HXK-overexpression also mildly protected plants against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000, a response that was accompanied by increased H2O2 production and elevated PR1 gene expression. These results demonstrate that higher levels of hexokinase confer improved resistance to MV-induced oxidative stress and pathogen infection.

Original languageEnglish
Pages (from-to)341-346
Number of pages6
JournalJournal of Plant Biology
Volume51
Issue number5
DOIs
Publication statusPublished - 2008 Aug 30

Fingerprint

hexokinase
oxidative stress
pathogens
paraquat
infection
Alternaria brassicicola
Pseudomonas syringae pv. tomato
Nicotiana benthamiana
biotic stress
salicylic acid
abiotic stress
mitochondria
apoptosis
Arabidopsis
gene expression
genes

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Sarowar, Sujon ; Lee, Jae Yong ; Ahn, Eu Ree ; Pai, Hyun Sook. / A role of hexokinases in plant resistance to oxidative stress and pathogen infection. In: Journal of Plant Biology. 2008 ; Vol. 51, No. 5. pp. 341-346.
@article{29872629b9634de9b0febc1399ec3e60,
title = "A role of hexokinases in plant resistance to oxidative stress and pathogen infection",
abstract = "Previously, we reported that mitochondria-associated hexokinases are active in controlling programmed cell death in plants (Plant Cell 18, 2341-2355). Here, we investigated their role under abiotic- and biotic-stress conditions. Expression of NbHxk1, a Nicotiana benthamiana hexokinase gene, was stimulated by treatment with salicylic acid or methyl viologen (MV), and was also up-regulated by pathogen infection. In response to MV-induced oxidative stress, NbHxk1-silenced plants exhibited increased susceptibility, while the HXK1- and HXK2-overexpressing Arabidopsis plants had enhanced tolerance. Moreover, those overexpressing plants showed greater resistance to the necrotrophic fungal pathogen Alternaria brassicicola. HXK-overexpression also mildly protected plants against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000, a response that was accompanied by increased H2O2 production and elevated PR1 gene expression. These results demonstrate that higher levels of hexokinase confer improved resistance to MV-induced oxidative stress and pathogen infection.",
author = "Sujon Sarowar and Lee, {Jae Yong} and Ahn, {Eu Ree} and Pai, {Hyun Sook}",
year = "2008",
month = "8",
day = "30",
doi = "10.1007/BF03036136",
language = "English",
volume = "51",
pages = "341--346",
journal = "Journal of Plant Biology",
issn = "1226-9239",
publisher = "Springer New York",
number = "5",

}

A role of hexokinases in plant resistance to oxidative stress and pathogen infection. / Sarowar, Sujon; Lee, Jae Yong; Ahn, Eu Ree; Pai, Hyun Sook.

In: Journal of Plant Biology, Vol. 51, No. 5, 30.08.2008, p. 341-346.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A role of hexokinases in plant resistance to oxidative stress and pathogen infection

AU - Sarowar, Sujon

AU - Lee, Jae Yong

AU - Ahn, Eu Ree

AU - Pai, Hyun Sook

PY - 2008/8/30

Y1 - 2008/8/30

N2 - Previously, we reported that mitochondria-associated hexokinases are active in controlling programmed cell death in plants (Plant Cell 18, 2341-2355). Here, we investigated their role under abiotic- and biotic-stress conditions. Expression of NbHxk1, a Nicotiana benthamiana hexokinase gene, was stimulated by treatment with salicylic acid or methyl viologen (MV), and was also up-regulated by pathogen infection. In response to MV-induced oxidative stress, NbHxk1-silenced plants exhibited increased susceptibility, while the HXK1- and HXK2-overexpressing Arabidopsis plants had enhanced tolerance. Moreover, those overexpressing plants showed greater resistance to the necrotrophic fungal pathogen Alternaria brassicicola. HXK-overexpression also mildly protected plants against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000, a response that was accompanied by increased H2O2 production and elevated PR1 gene expression. These results demonstrate that higher levels of hexokinase confer improved resistance to MV-induced oxidative stress and pathogen infection.

AB - Previously, we reported that mitochondria-associated hexokinases are active in controlling programmed cell death in plants (Plant Cell 18, 2341-2355). Here, we investigated their role under abiotic- and biotic-stress conditions. Expression of NbHxk1, a Nicotiana benthamiana hexokinase gene, was stimulated by treatment with salicylic acid or methyl viologen (MV), and was also up-regulated by pathogen infection. In response to MV-induced oxidative stress, NbHxk1-silenced plants exhibited increased susceptibility, while the HXK1- and HXK2-overexpressing Arabidopsis plants had enhanced tolerance. Moreover, those overexpressing plants showed greater resistance to the necrotrophic fungal pathogen Alternaria brassicicola. HXK-overexpression also mildly protected plants against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000, a response that was accompanied by increased H2O2 production and elevated PR1 gene expression. These results demonstrate that higher levels of hexokinase confer improved resistance to MV-induced oxidative stress and pathogen infection.

UR - http://www.scopus.com/inward/record.url?scp=54249155718&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54249155718&partnerID=8YFLogxK

U2 - 10.1007/BF03036136

DO - 10.1007/BF03036136

M3 - Article

AN - SCOPUS:54249155718

VL - 51

SP - 341

EP - 346

JO - Journal of Plant Biology

JF - Journal of Plant Biology

SN - 1226-9239

IS - 5

ER -