A sample preparation technique using biocompatible composites for biomedical applications

Huifang Liu, Geun Su Noh, Yange Luan, Zhen Qiao, Bonhan Koo, Yoon Ok Jang, Yong Shin

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential for sensitive diagnostics. In this study, a cucurbit[6]uril (CB) supermolecular decorated amine-functionalized diatom (DA) composite was fabricated to support efficient sample enrichment and in situ nucleic acid preparation from enriched pathogens and cells. CB was introduced to enhance the rate and effectiveness of pathogen absorption using the CB–DA composite. This novel CB–DA composite achieved a capture efficiency of approximately 90% at an Escherichia coli concentration of 106 CFU/mL within 3 min. Real-time PCR analyses of DNA samples recovered using the CB–DA enrichment system showed a four-fold increase in the early amplification signal strength, and this effective method for capturing nucleic acid might be useful for preparing samples for diagnostic systems.

Original languageEnglish
Article number1321
JournalMolecules
Volume24
Issue number7
DOIs
Publication statusPublished - 2019 Apr 3

Bibliographical note

Funding Information:
Funding: This study was supported by the Ministry of Science, ICT, and Future Planning (MSIP) through the National Research Foundation of Korea (NRF) (2017R1A2B4005288), Republic of Korea.

Publisher Copyright:
© 2019 by the authors.

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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