A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA synthetase

Yoshiaki Kise; Sang Won Lee; Sang Gyu Park; Shuya Fukai; Toru Sengoku; Ryohei Ishii; Shigeyuki Yokoyama; Sunghoon Kim; Osamu Nureki

doi:10.1038/nsmb722

A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA synthetase

Yoshiaki Kise, Sang Won Lee, Sang Gyu Park, Shuya Fukai, Toru Sengoku, Ryohei Ishii, Shigeyuki Yokoyama, Sunghoon Kim, Osamu Nureki

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

Human tryptophanyl-tRNA synthetase (TrpRS) is secreted into the extracellular region of vascular endothelial cells. The splice variant form (mini TrpRS) functions in vascular endothelial cell apoptosis as an angiostatic cytokine. In contrast, the closely related human tyrosyl-tRNA synthetase (TyrRS) functions as an angiogenic cytokine in its truncated form (mini TyrRS). Here, we determined the crystal structure of human mini TrpRS at a resolution of 2.3 Å and compared the structure with those of prokaryotic TrpRS and human mini TyrRS. Deletion of the tRNA anticodon-binding (TAB) domain insertion, consisting of eight residues in the human TrpRS, abolished the enzyme's apoptotic activity for endothelial cells, whereas its translational catalysis and cell-binding activities remained unchanged. Thus, we have identified the inserted peptide motif that activates the angiostatic signaling.

Original language	English
Pages (from-to)	149-156
Number of pages	8
Journal	Nature Structural and Molecular Biology
Volume	11
Issue number	2
DOIs	https://doi.org/10.1038/nsmb722
Publication status	Published - 2004 Feb

Bibliographical note

Funding Information:
We thank M. Ibba (Ohio State University) for helpful discussions during the manuscript preparation. We also thank M. Kawamoto and H. Sakai (Japanese Synchrotron Radiation Research Institute (JASRI)) for their help in data collection at SPring-8. This work was supported by a PRESTO Program grant from Japan Science and Technology and a Naito Foundation grant to O.N., and by a grant for National Creative Research Initiatives from the Ministry of Science and Technology, Korea to S.K.

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Structural Biology
Molecular Biology

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title = "A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA synthetase",

abstract = "Human tryptophanyl-tRNA synthetase (TrpRS) is secreted into the extracellular region of vascular endothelial cells. The splice variant form (mini TrpRS) functions in vascular endothelial cell apoptosis as an angiostatic cytokine. In contrast, the closely related human tyrosyl-tRNA synthetase (TyrRS) functions as an angiogenic cytokine in its truncated form (mini TyrRS). Here, we determined the crystal structure of human mini TrpRS at a resolution of 2.3 {\AA} and compared the structure with those of prokaryotic TrpRS and human mini TyrRS. Deletion of the tRNA anticodon-binding (TAB) domain insertion, consisting of eight residues in the human TrpRS, abolished the enzyme's apoptotic activity for endothelial cells, whereas its translational catalysis and cell-binding activities remained unchanged. Thus, we have identified the inserted peptide motif that activates the angiostatic signaling.",

author = "Yoshiaki Kise and Lee, {Sang Won} and Park, {Sang Gyu} and Shuya Fukai and Toru Sengoku and Ryohei Ishii and Shigeyuki Yokoyama and Sunghoon Kim and Osamu Nureki",

note = "Funding Information: We thank M. Ibba (Ohio State University) for helpful discussions during the manuscript preparation. We also thank M. Kawamoto and H. Sakai (Japanese Synchrotron Radiation Research Institute (JASRI)) for their help in data collection at SPring-8. This work was supported by a PRESTO Program grant from Japan Science and Technology and a Naito Foundation grant to O.N., and by a grant for National Creative Research Initiatives from the Ministry of Science and Technology, Korea to S.K.",

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AU - Sengoku, Toru

AU - Ishii, Ryohei

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AU - Kim, Sunghoon

AU - Nureki, Osamu

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A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA synthetase

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