Signature-tagged mutagenesis (STM) is a high-throughput genetic technique that can be used to investigate the function of genes by constructing a large number of mutant strains with unique DNA identification tags, pooling them, and screening them for a particular phenotypic trait. STM was first designed for the identification of genes that contribute to the virulence or infectivity of a pathogen in its host. Recently, this method has also been applied for the identification of mutants with specific phenotypes, such as antifungal drug resistance and proliferation. In the present study, we describe an STM method for the identification of genes contributing to the infectivity of Cryptococcus neoformans using a mutant library, in which each strain was tagged with a unique DNA sequence.
Bibliographical noteFunding Information:
This work was supported by National Research Foundation of Korea grants (2018R1A5A1025077 to Y.-S. Bahn; 2018-R1C1B6009031 to K.-T.L.) from the Ministry of Science and Information and Communications Technologies (ICT) and by the Strategic Initiative for Microbiomes in Agriculture and Food (918012-4 to Y.-S.B.) funded by the Ministry of Agriculture, Food and Rural Affairs. This work was also supported by the Nuclear R&D program of Ministry of Science and ICT (Republic of Korea) (to K.-W. Jung) and the Brain Korea 21 (BK21) PLUS program.
© 2020, The Microbiological Society of Korea.
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology