A simple and efficient method for the isolation of total RNA from the fission yeast Schizosaccharomyces pombe

Yeun Kyu Jang, Y. H. Jin, M. J. Kim, R. H. Seong, S. H. Hong, S. D. Park

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A simple and efficient procedure was described for the isolation of total RNA from the fission yeast Schizosaccharomyces pombe. The present study demonstrated that the quality and the quantity of S. pombe RNA were increased by substituting phenol/chloroform mixture for phenol as a deproteinizing agent in the first vortexing step and using an ice bath instead of a dry ice-ethanol bath in the freezing step. Additionally, this protocol had the advantage of extracting total RNA without any degradation of S. pombe cells. Furthermore, the high amounts and quality of RNA extracted by this modified procedure enabled us to perform some experiments such as Northern blot, S1 mapping, primer extension, and reverse transcriptase reaction-polymerase chain reaction (RT-PCR) without further RNA purification. We suggest that this procedure is very useful to analyse primary structures and steady-state levels of RNA from S. pombe.

Original languageEnglish
Pages (from-to)339-344
Number of pages6
JournalBiochemistry and Molecular Biology International
Volume37
Issue number2
Publication statusPublished - 1995 Dec 1

Fingerprint

Schizosaccharomyces
Yeast
RNA
Phenol
Baths
Dry Ice
Polymerase chain reaction
RNA-Directed DNA Polymerase
Ice
Chloroform
Freezing
Northern Blotting
Purification
Ethanol
Degradation
Polymerase Chain Reaction
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Jang, Yeun Kyu ; Jin, Y. H. ; Kim, M. J. ; Seong, R. H. ; Hong, S. H. ; Park, S. D. / A simple and efficient method for the isolation of total RNA from the fission yeast Schizosaccharomyces pombe. In: Biochemistry and Molecular Biology International. 1995 ; Vol. 37, No. 2. pp. 339-344.
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A simple and efficient method for the isolation of total RNA from the fission yeast Schizosaccharomyces pombe. / Jang, Yeun Kyu; Jin, Y. H.; Kim, M. J.; Seong, R. H.; Hong, S. H.; Park, S. D.

In: Biochemistry and Molecular Biology International, Vol. 37, No. 2, 01.12.1995, p. 339-344.

Research output: Contribution to journalArticle

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