A simple and rapid fungal DNA isolation assay based on ZnO nanoparticles for the diagnosis of invasive aspergillosis

Zhen Qiao, Huifang Liu, Geun Su Noh, Bonhan Koo, Qingshuang Zou, Kyusik Yun, Yoon Ok Jang, Sung Han Kim, Yong Shin

Research output: Contribution to journalArticlepeer-review

Abstract

Invasive aspergillosis (IA) is an important cause of morbidity and mortality among immunocompromised people. Imaging and specimen tests used in the clinical diagnosis of aspergillosis with weak and indistinct defects leads to delay in the treatment of early aspergillosis patients. The developing molecular techniques provide a new method for the aspergillosis diagnosis. However, the existing methods are complex, time-consuming and may even be potentially hazardous. In this study, we developed a simple and rapid Aspergillus fumigatus spores DNA isolation assay using synthesized zinc oxide (ZnO). ZnO nanoparticles were used to take the place of the traditional commercial lysis buffer. The quality and quantity of the extracted DNA were sufficient for further diagnostics with polymerase chain reaction (PCR) analysis. This method offers easy, green, and economic alternative DNA isolation for the diagnosis of invasive aspergillosis.

Original languageEnglish
Article number515
JournalMicromachines
Volume11
Issue number5
DOIs
Publication statusPublished - 2020 May 1

Bibliographical note

Funding Information:
Author Contributions: conceptualization, S.-H.K. and Y.S.; methodology, Z.Q. and H.L.; validation, Z.Q. and AHu.Lth.;o irnCveosntitgriabtiuotnio, nZs.Q: C. aonndce Hpt.uLa.;l irzeastoiuornc,eSs.,-HG..SK.N. a.n, dB.KY..S, .Q; m.Ze.,t hKo.dYo., loangdy, YZ..OQ..J.a; nddatHa .cLu.;ravtaiolind,aZti.oQn., aZn.Qd.Ha.nLd.; writing—original draft preparation, Z.Q. and H.L.; writing—review and editing, Z.Q., H.L., S.-H.K. and Y.S.; writing—original draft preparation, Z.Q. and H.L.; writing—review and editing, Z.Q., H.L., S.-H.K. and Y.S.; supervision, Y.S.; funding acquisition, S.-H.K. and Y.S. supervision, Y.S.; funding acquisition, S.-H.K. and Y.S. All authors have read and agreed to the published version Funding: This study was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic Hofe aKlothreIan d(HusIt2r0yCD00ev73e)lo, panmde naltsIon sstuitpupteor(tKeHd IbDyI)t,hfeu Mndiendisbtryy tohfe SMciienniscter,y ICofTH aenadlt FhuatnudreW Pellafnarnei,nRge (pMuSbIliPc) otfhKroourgeah (HI20C0073), and also supported by the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation of Korea (NRF) (2020R1A2C2007148). Conflicts of Interest: The authors declare no conflict of interest. Conflicts of Interest: The authors declare no conflict of interest.

Publisher Copyright:
© 2020 by the authors.

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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