A simple and rapid fungal DNA isolation assay based on ZnO nanoparticles for the diagnosis of invasive aspergillosis

Zhen Qiao; Huifang Liu; Geun Su Noh; Bonhan Koo; Qingshuang Zou; Kyusik Yun; Yoon Ok Jang; Sung Han Kim; Yong Shin

doi:10.3390/mi11050515

A simple and rapid fungal DNA isolation assay based on ZnO nanoparticles for the diagnosis of invasive aspergillosis

Zhen Qiao, Huifang Liu, Geun Su Noh, Bonhan Koo, Qingshuang Zou, Kyusik Yun, Yoon Ok Jang, Sung Han Kim, Yong Shin

Division of Life Sciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Invasive aspergillosis (IA) is an important cause of morbidity and mortality among immunocompromised people. Imaging and specimen tests used in the clinical diagnosis of aspergillosis with weak and indistinct defects leads to delay in the treatment of early aspergillosis patients. The developing molecular techniques provide a new method for the aspergillosis diagnosis. However, the existing methods are complex, time-consuming and may even be potentially hazardous. In this study, we developed a simple and rapid Aspergillus fumigatus spores DNA isolation assay using synthesized zinc oxide (ZnO). ZnO nanoparticles were used to take the place of the traditional commercial lysis buffer. The quality and quantity of the extracted DNA were sufficient for further diagnostics with polymerase chain reaction (PCR) analysis. This method offers easy, green, and economic alternative DNA isolation for the diagnosis of invasive aspergillosis.

Original language	English
Article number	515
Journal	Micromachines
Volume	11
Issue number	5
DOIs	https://doi.org/10.3390/mi11050515
Publication status	Published - 2020 May 1

Bibliographical note

Funding Information:
Author Contributions: conceptualization, S.-H.K. and Y.S.; methodology, Z.Q. and H.L.; validation, Z.Q. and AHu.Lth.;o irnCveosntitgriabtiuotnio, nZs.Q: C. aonndce Hpt.uLa.;l irzeastoiuornc,eSs.,-HG..SK.N. a.n, dB.KY..S, .Q; m.Ze.,t hKo.dYo., loangdy, YZ..OQ..J.a; nddatHa .cLu.;ravtaiolind,aZti.oQn., aZn.Qd.Ha.nLd.; writing—original draft preparation, Z.Q. and H.L.; writing—review and editing, Z.Q., H.L., S.-H.K. and Y.S.; writing—original draft preparation, Z.Q. and H.L.; writing—review and editing, Z.Q., H.L., S.-H.K. and Y.S.; supervision, Y.S.; funding acquisition, S.-H.K. and Y.S. supervision, Y.S.; funding acquisition, S.-H.K. and Y.S. All authors have read and agreed to the published version Funding: This study was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic Hofe aKlothreIan d(HusIt2r0yCD00ev73e)lo, panmde naltsIon sstuitpupteor(tKeHd IbDyI)t,hfeu Mndiendisbtryy tohfe SMciienniscter,y ICofTH aenadlt FhuatnudreW Pellafnarnei,nRge (pMuSbIliPc) otfhKroourgeah (HI20C0073), and also supported by the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation of Korea (NRF) (2020R1A2C2007148). Conflicts of Interest: The authors declare no conflict of interest. Conflicts of Interest: The authors declare no conflict of interest.

Publisher Copyright:
© 2020 by the authors.

All Science Journal Classification (ASJC) codes

Control and Systems Engineering
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.3390/mi11050515

Cite this

@article{1c4355d076a94b4a98a0f33b12463e02,

title = "A simple and rapid fungal DNA isolation assay based on ZnO nanoparticles for the diagnosis of invasive aspergillosis",

abstract = "Invasive aspergillosis (IA) is an important cause of morbidity and mortality among immunocompromised people. Imaging and specimen tests used in the clinical diagnosis of aspergillosis with weak and indistinct defects leads to delay in the treatment of early aspergillosis patients. The developing molecular techniques provide a new method for the aspergillosis diagnosis. However, the existing methods are complex, time-consuming and may even be potentially hazardous. In this study, we developed a simple and rapid Aspergillus fumigatus spores DNA isolation assay using synthesized zinc oxide (ZnO). ZnO nanoparticles were used to take the place of the traditional commercial lysis buffer. The quality and quantity of the extracted DNA were sufficient for further diagnostics with polymerase chain reaction (PCR) analysis. This method offers easy, green, and economic alternative DNA isolation for the diagnosis of invasive aspergillosis.",

author = "Zhen Qiao and Huifang Liu and Noh, {Geun Su} and Bonhan Koo and Qingshuang Zou and Kyusik Yun and Jang, {Yoon Ok} and Kim, {Sung Han} and Yong Shin",

note = "Funding Information: Author Contributions: conceptualization, S.-H.K. and Y.S.; methodology, Z.Q. and H.L.; validation, Z.Q. and AHu.Lth.;o irnCveosntitgriabtiuotnio, nZs.Q: C. aonndce Hpt.uLa.;l irzeastoiuornc,eSs.,-HG..SK.N. a.n, dB.KY..S, .Q; m.Ze.,t hKo.dYo., loangdy, YZ..OQ..J.a; nddatHa .cLu.;ravtaiolind,aZti.oQn., aZn.Qd.Ha.nLd.; writing—original draft preparation, Z.Q. and H.L.; writing—review and editing, Z.Q., H.L., S.-H.K. and Y.S.; writing—original draft preparation, Z.Q. and H.L.; writing—review and editing, Z.Q., H.L., S.-H.K. and Y.S.; supervision, Y.S.; funding acquisition, S.-H.K. and Y.S. supervision, Y.S.; funding acquisition, S.-H.K. and Y.S. All authors have read and agreed to the published version Funding: This study was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic Hofe aKlothreIan d(HusIt2r0yCD00ev73e)lo, panmde naltsIon sstuitpupteor(tKeHd IbDyI)t,hfeu Mndiendisbtryy tohfe SMciienniscter,y ICofTH aenadlt FhuatnudreW Pellafnarnei,nRge (pMuSbIliPc) otfhKroourgeah (HI20C0073), and also supported by the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation of Korea (NRF) (2020R1A2C2007148). Conflicts of Interest: The authors declare no conflict of interest. Conflicts of Interest: The authors declare no conflict of interest. Publisher Copyright: {\textcopyright} 2020 by the authors.",

year = "2020",

month = may,

day = "1",

doi = "10.3390/mi11050515",

language = "English",

volume = "11",

journal = "Micromachines",

issn = "2072-666X",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "5",

}

TY - JOUR

T1 - A simple and rapid fungal DNA isolation assay based on ZnO nanoparticles for the diagnosis of invasive aspergillosis

AU - Qiao, Zhen

AU - Liu, Huifang

AU - Noh, Geun Su

AU - Koo, Bonhan

AU - Zou, Qingshuang

AU - Yun, Kyusik

AU - Jang, Yoon Ok

AU - Kim, Sung Han

AU - Shin, Yong

N1 - Funding Information: Author Contributions: conceptualization, S.-H.K. and Y.S.; methodology, Z.Q. and H.L.; validation, Z.Q. and AHu.Lth.;o irnCveosntitgriabtiuotnio, nZs.Q: C. aonndce Hpt.uLa.;l irzeastoiuornc,eSs.,-HG..SK.N. a.n, dB.KY..S, .Q; m.Ze.,t hKo.dYo., loangdy, YZ..OQ..J.a; nddatHa .cLu.;ravtaiolind,aZti.oQn., aZn.Qd.Ha.nLd.; writing—original draft preparation, Z.Q. and H.L.; writing—review and editing, Z.Q., H.L., S.-H.K. and Y.S.; writing—original draft preparation, Z.Q. and H.L.; writing—review and editing, Z.Q., H.L., S.-H.K. and Y.S.; supervision, Y.S.; funding acquisition, S.-H.K. and Y.S. supervision, Y.S.; funding acquisition, S.-H.K. and Y.S. All authors have read and agreed to the published version Funding: This study was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic Hofe aKlothreIan d(HusIt2r0yCD00ev73e)lo, panmde naltsIon sstuitpupteor(tKeHd IbDyI)t,hfeu Mndiendisbtryy tohfe SMciienniscter,y ICofTH aenadlt FhuatnudreW Pellafnarnei,nRge (pMuSbIliPc) otfhKroourgeah (HI20C0073), and also supported by the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation of Korea (NRF) (2020R1A2C2007148). Conflicts of Interest: The authors declare no conflict of interest. Conflicts of Interest: The authors declare no conflict of interest. Publisher Copyright: © 2020 by the authors.

PY - 2020/5/1

Y1 - 2020/5/1

N2 - Invasive aspergillosis (IA) is an important cause of morbidity and mortality among immunocompromised people. Imaging and specimen tests used in the clinical diagnosis of aspergillosis with weak and indistinct defects leads to delay in the treatment of early aspergillosis patients. The developing molecular techniques provide a new method for the aspergillosis diagnosis. However, the existing methods are complex, time-consuming and may even be potentially hazardous. In this study, we developed a simple and rapid Aspergillus fumigatus spores DNA isolation assay using synthesized zinc oxide (ZnO). ZnO nanoparticles were used to take the place of the traditional commercial lysis buffer. The quality and quantity of the extracted DNA were sufficient for further diagnostics with polymerase chain reaction (PCR) analysis. This method offers easy, green, and economic alternative DNA isolation for the diagnosis of invasive aspergillosis.

AB - Invasive aspergillosis (IA) is an important cause of morbidity and mortality among immunocompromised people. Imaging and specimen tests used in the clinical diagnosis of aspergillosis with weak and indistinct defects leads to delay in the treatment of early aspergillosis patients. The developing molecular techniques provide a new method for the aspergillosis diagnosis. However, the existing methods are complex, time-consuming and may even be potentially hazardous. In this study, we developed a simple and rapid Aspergillus fumigatus spores DNA isolation assay using synthesized zinc oxide (ZnO). ZnO nanoparticles were used to take the place of the traditional commercial lysis buffer. The quality and quantity of the extracted DNA were sufficient for further diagnostics with polymerase chain reaction (PCR) analysis. This method offers easy, green, and economic alternative DNA isolation for the diagnosis of invasive aspergillosis.

UR - http://www.scopus.com/inward/record.url?scp=85085939781&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85085939781&partnerID=8YFLogxK

U2 - 10.3390/mi11050515

DO - 10.3390/mi11050515

M3 - Article

AN - SCOPUS:85085939781

SN - 2072-666X

VL - 11

JO - Micromachines

JF - Micromachines

IS - 5

M1 - 515

ER -

A simple and rapid fungal DNA isolation assay based on ZnO nanoparticles for the diagnosis of invasive aspergillosis

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this