A single-stranded telomere binding protein in the nematode Caenorhabditis elegans

Soo Young Yi, Kyu Sang Joeng, Jai Uk Kweon, Jin Won Cho, In Kwon Chung, Junho Lee

Research output: Contribution to journalArticle

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Abstract

We identified and characterized a protein (STB-1) from the nuclear extract of Caenorhabditis elegans that specifically binds single-stranded telomere DNA sequences, but not the corresponding RNA sequences. STB-1 binding activity is specific to the nematode telomere, but not to the human or plant telomere. STB-1 requires the core nucleotides of GCTTAGG and three spacer nucleotides in front of them for binding. While any single nucleotide change in the core sequence abolishes binding, the spacer nucleotides tolerate substitution. STB-1 was determined to be a basic protein of 45 kDa by Southwestern analyses. STB-1 forms a stable complex with DNA once bound to the telomere.

Original languageEnglish
Pages (from-to)301-306
Number of pages6
JournalFEBS Letters
Volume505
Issue number2
DOIs
Publication statusPublished - 2001 Sep 14

Fingerprint

Telomere-Binding Proteins
Telomere
Caenorhabditis elegans
Nucleotides
Single-Stranded DNA
DNA sequences
Proteins
Substitution reactions
RNA
DNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Yi, Soo Young ; Joeng, Kyu Sang ; Kweon, Jai Uk ; Cho, Jin Won ; Chung, In Kwon ; Lee, Junho. / A single-stranded telomere binding protein in the nematode Caenorhabditis elegans. In: FEBS Letters. 2001 ; Vol. 505, No. 2. pp. 301-306.
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A single-stranded telomere binding protein in the nematode Caenorhabditis elegans. / Yi, Soo Young; Joeng, Kyu Sang; Kweon, Jai Uk; Cho, Jin Won; Chung, In Kwon; Lee, Junho.

In: FEBS Letters, Vol. 505, No. 2, 14.09.2001, p. 301-306.

Research output: Contribution to journalArticle

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