A single-stranded telomere binding protein in the nematode Caenorhabditis elegans

Soo Young Yi; Kyu Sang Joeng; Jai Uk Kweon; Jin Won Cho; In Kwon Chung; Junho Lee

doi:10.1016/S0014-5793(01)02821-6

A single-stranded telomere binding protein in the nematode Caenorhabditis elegans

Soo Young Yi, Kyu Sang Joeng, Jai Uk Kweon, Jin Won Cho, In Kwon Chung, Junho Lee

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We identified and characterized a protein (STB-1) from the nuclear extract of Caenorhabditis elegans that specifically binds single-stranded telomere DNA sequences, but not the corresponding RNA sequences. STB-1 binding activity is specific to the nematode telomere, but not to the human or plant telomere. STB-1 requires the core nucleotides of GCTTAGG and three spacer nucleotides in front of them for binding. While any single nucleotide change in the core sequence abolishes binding, the spacer nucleotides tolerate substitution. STB-1 was determined to be a basic protein of 45 kDa by Southwestern analyses. STB-1 forms a stable complex with DNA once bound to the telomere.

Original language	English
Pages (from-to)	301-306
Number of pages	6
Journal	FEBS Letters
Volume	505
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(01)02821-6
Publication status	Published - 2001 Sept 14

Bibliographical note

Funding Information:
We thank M.S. Choi and lab members for critical reading and advice. This work was supported by Korea Research Foundation Grant KRF-2000-2-0821 to I.K.C. and J.H.L.

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(01)02821-6

Cite this

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title = "A single-stranded telomere binding protein in the nematode Caenorhabditis elegans",

abstract = "We identified and characterized a protein (STB-1) from the nuclear extract of Caenorhabditis elegans that specifically binds single-stranded telomere DNA sequences, but not the corresponding RNA sequences. STB-1 binding activity is specific to the nematode telomere, but not to the human or plant telomere. STB-1 requires the core nucleotides of GCTTAGG and three spacer nucleotides in front of them for binding. While any single nucleotide change in the core sequence abolishes binding, the spacer nucleotides tolerate substitution. STB-1 was determined to be a basic protein of 45 kDa by Southwestern analyses. STB-1 forms a stable complex with DNA once bound to the telomere.",

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AU - Joeng, Kyu Sang

AU - Kweon, Jai Uk

AU - Cho, Jin Won

AU - Chung, In Kwon

AU - Lee, Junho

N1 - Funding Information: We thank M.S. Choi and lab members for critical reading and advice. This work was supported by Korea Research Foundation Grant KRF-2000-2-0821 to I.K.C. and J.H.L.

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N2 - We identified and characterized a protein (STB-1) from the nuclear extract of Caenorhabditis elegans that specifically binds single-stranded telomere DNA sequences, but not the corresponding RNA sequences. STB-1 binding activity is specific to the nematode telomere, but not to the human or plant telomere. STB-1 requires the core nucleotides of GCTTAGG and three spacer nucleotides in front of them for binding. While any single nucleotide change in the core sequence abolishes binding, the spacer nucleotides tolerate substitution. STB-1 was determined to be a basic protein of 45 kDa by Southwestern analyses. STB-1 forms a stable complex with DNA once bound to the telomere.

AB - We identified and characterized a protein (STB-1) from the nuclear extract of Caenorhabditis elegans that specifically binds single-stranded telomere DNA sequences, but not the corresponding RNA sequences. STB-1 binding activity is specific to the nematode telomere, but not to the human or plant telomere. STB-1 requires the core nucleotides of GCTTAGG and three spacer nucleotides in front of them for binding. While any single nucleotide change in the core sequence abolishes binding, the spacer nucleotides tolerate substitution. STB-1 was determined to be a basic protein of 45 kDa by Southwestern analyses. STB-1 forms a stable complex with DNA once bound to the telomere.

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A single-stranded telomere binding protein in the nematode Caenorhabditis elegans

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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