A single tube assay based on dual-electrostatic interaction strategy for rapid and ultrasensitive pathogenic bacteria detection

Feixiong Chen, Dao Thi Thuy Nguyen, Yong Shin, Tae Yoon Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In pursuit of integration of bacteria enrichment and DNA extraction in a single tube serving to pathogenic bacteria diagnosis, a dilemma exists between the strongly electrostatic interaction (EI) in bacteria capture and the weakly EI in DNA extraction. We herein designed a single tube assay based on dual-EI strategies for the capture of pathogenic bacteria and DNA extraction from captured bacteria for compatible qPCR detection. Based on this assay, we evaluated the performance of detection with Salmonella enterica (S. enterica) in human urine (10 mL). The detection limit of 5 colony-forming unit (CFU)/10mL is achieved. This assay offered a simple and rapid sample preparation tool for highly sensitive detection of pathogenic bacteria from raw samples.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages1574-1575
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: 2019 Oct 272019 Oct 31

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Country/TerritorySwitzerland
CityBasel
Period19/10/2719/10/31

Bibliographical note

Funding Information:
This work was supported by a research fund of Chungnam National University.

Publisher Copyright:
© 2019 CBMS-0001.

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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