A small molecule inhibitor for ATPase activity of Hsp70 and Hsc70 enhances the immune response to protein antigens

Kyung Hwa Baek, Haiying Zhang, Bo Ryeong Lee, Young-Guen Kwon, Sang-Jun Ha, Injae Shin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The ATPase activities of Hsp70 and Hsc70 are known to be responsible for regulation of various biological processes. However, little is known about the roles of Hsp70 and Hsc70 in modulation of immune responses to antigens. In the present study, we investigated the effect of apoptozole (Az), a small molecule inhibitor of Hsp70 and Hsc70, on immune responses to protein antigens. The results show that mice administered with both protein antigen and Az produce more antibodies than those treated with antigen alone, showing that Az enhances immune responses to administered antigens. Treatment of mice with Az elicits production of antibodies with a high IgG2c/IgG1 ratio and stimulates the release of Th1 and Th2-type cytokines, suggesting that Az activates the Th1 and Th2 immune responses. The observations made in the present study suggest that inhibition of Hsp70 and Hsc70 activities could be a novel strategy designing small molecule-based adjuvants in protein vaccines.

Original languageEnglish
Article number17642
JournalScientific reports
Volume5
DOIs
Publication statusPublished - 2015 Dec 3

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Adenosine Triphosphatases
Antigens
Proteins
Biological Phenomena
Histocompatibility Antigens Class II
Antibody Formation
Vaccines
Immunoglobulin G
apoptozole
Cytokines
Antibodies

All Science Journal Classification (ASJC) codes

  • General

Cite this

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abstract = "The ATPase activities of Hsp70 and Hsc70 are known to be responsible for regulation of various biological processes. However, little is known about the roles of Hsp70 and Hsc70 in modulation of immune responses to antigens. In the present study, we investigated the effect of apoptozole (Az), a small molecule inhibitor of Hsp70 and Hsc70, on immune responses to protein antigens. The results show that mice administered with both protein antigen and Az produce more antibodies than those treated with antigen alone, showing that Az enhances immune responses to administered antigens. Treatment of mice with Az elicits production of antibodies with a high IgG2c/IgG1 ratio and stimulates the release of Th1 and Th2-type cytokines, suggesting that Az activates the Th1 and Th2 immune responses. The observations made in the present study suggest that inhibition of Hsp70 and Hsc70 activities could be a novel strategy designing small molecule-based adjuvants in protein vaccines.",
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A small molecule inhibitor for ATPase activity of Hsp70 and Hsc70 enhances the immune response to protein antigens. / Baek, Kyung Hwa; Zhang, Haiying; Lee, Bo Ryeong; Kwon, Young-Guen; Ha, Sang-Jun; Shin, Injae.

In: Scientific reports, Vol. 5, 17642, 03.12.2015.

Research output: Contribution to journalArticle

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AU - Baek, Kyung Hwa

AU - Zhang, Haiying

AU - Lee, Bo Ryeong

AU - Kwon, Young-Guen

AU - Ha, Sang-Jun

AU - Shin, Injae

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