A small molecule inhibitor of ATPase activity of HSP70 induces apoptosis and has antitumor activities

Sung Kyun Ko, Jiyeon Kim, Deuk Chae Na, Sookil Park, Seong Hyun Park, Ji Young Hyun, Kyung Hwa Baek, Nam Doo Kim, Nak Kyoon Kim, Young Nyun Park, Kiwon Song, Injae Shin

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42 Citations (Scopus)

Abstract

Summary The heat shock protein HSP70 plays antiapoptotic and oncogenic roles, and thus its inhibition has been recognized as a potential avenue for anticancer therapy. Here we describe the small molecule, apoptozole (Az), which inhibits the ATPase activity of HSP70 by binding to its ATPase domain and, as a result, induces an array of apoptotic phenotypes in cancer cells. Affinity chromatography provides evidence that Az binds HSP70 but not other types of heat shock proteins including HSP40, HSP60, and HSP90. We also demonstrate that Az induces cancer cell death via caspase-dependent apoptosis by disrupting the interaction of HSP70 with APAF-1. Animal studies indicate that Az treatment retards tumor growth in a xenograft mouse model without affecting mouse viability. These studies suggest that Az will aid the development of new cancer therapies and serve as a chemical probe to gain a better understanding of the diverse functions of HSP70.

Original languageEnglish
Article number3008
Pages (from-to)391-403
Number of pages13
JournalChemistry and Biology
Volume22
Issue number3
DOIs
Publication statusPublished - 2015 Mar 19

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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    Ko, S. K., Kim, J., Na, D. C., Park, S., Park, S. H., Hyun, J. Y., Baek, K. H., Kim, N. D., Kim, N. K., Park, Y. N., Song, K., & Shin, I. (2015). A small molecule inhibitor of ATPase activity of HSP70 induces apoptosis and has antitumor activities. Chemistry and Biology, 22(3), 391-403. [3008]. https://doi.org/10.1016/j.chembiol.2015.02.004