A smartphone-based chip-scale microscope using ambient illumination

Seung Ah Lee, Changhuei Yang

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Portable chip-scale microscopy devices can potentially address various imaging needs in mobile healthcare and environmental monitoring. Here, we demonstrate the adaptation of a smartphone's camera to function as a compact lensless microscope. Unlike other chip-scale microscopy schemes, this method uses ambient illumination as its light source and does not require the incorporation of a dedicated light source. The method is based on the shadow imaging technique where the sample is placed on the surface of the image sensor, which captures direct shadow images under illumination. To improve the image resolution beyond the pixel size, we perform pixel super-resolution reconstruction with multiple images at different angles of illumination, which are captured while the user is manually tilting the device around any ambient light source, such as the sun or a lamp. The lensless imaging scheme allows for sub-micron resolution imaging over an ultra-wide field-of-view (FOV). Image acquisition and reconstruction are performed on the device using a custom-built Android application, constructing a stand-alone imaging device for field applications. We discuss the construction of the device using a commercial smartphone and demonstrate the imaging capabilities of our system.

Original languageEnglish
Pages (from-to)3056-3063
Number of pages8
JournalLab on a Chip
Volume14
Issue number16
DOIs
Publication statusPublished - 2014 Aug 21

Fingerprint

Smartphones
Lighting
Microscopes
Imaging techniques
Equipment and Supplies
Light sources
Light
Microscopy
Microscopic examination
Pixels
Computer-Assisted Image Processing
Environmental Monitoring
Solar System
Image acquisition
Image resolution
Image reconstruction
Electric lamps
Image sensors
Sun
Smartphone

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Lee, Seung Ah ; Yang, Changhuei. / A smartphone-based chip-scale microscope using ambient illumination. In: Lab on a Chip. 2014 ; Vol. 14, No. 16. pp. 3056-3063.
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A smartphone-based chip-scale microscope using ambient illumination. / Lee, Seung Ah; Yang, Changhuei.

In: Lab on a Chip, Vol. 14, No. 16, 21.08.2014, p. 3056-3063.

Research output: Contribution to journalArticle

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