Abstract
The mechanism of cell adhesion to biomaterials or components of the extracellular matrix is an important topic in the field of tissue engineering and related biotechnological processes. Many factors affect cell adhesion, and many biochemical and biological studies have attempted to identify their roles in the adhesion mechanism. Systematic studies of this nature require quantification of the adhesive force of a cell to identify the effect of a specific factor. However, most studies of cell adhesive force have used qualitative approaches. We propose a new technique for quantifying the force by which cells adhere to various biomaterial surfaces, which utilizes the relationship between the deflection of a cantilever beam and the required force. A micropipette was used as the cantilever beam. This technique was used to measure the attachment forces of chondrocytes seeded on three different biodegradable polymers commonly used in tissue engineering and medicine: poly E-carprolactone (PCL), poly(L-lactide) (PLLA) and poly(lactic-co-glycolic acid) (PGLA, L/G = 75: 25). The bond between the cells and the three polymers was evaluated using the quantified adhesive forces. The adhesive forces were also measured 8, 12, 24 h and 5 days after seeding the chondrocytes on the polymer surfaces. Results of statistical analysis showed that the cells attached to the PLLA had the strongest average attachment force for up to 24 h after seeding (P < 0.05).
| Original language | English |
|---|---|
| Pages (from-to) | 1311-1321 |
| Number of pages | 11 |
| Journal | Journal of Biomaterials Science, Polymer Edition |
| Volume | 14 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2003 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Bioengineering
- Biomaterials
- Biomedical Engineering
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A study of compatibility between cells and biopolymeric surfaces through quantitative measurements of adhesive forces. / Kim, Young Jick; Shin, Jung Woog; Park, Ki Dong; Lee, Jin Woo; Yui, Nobuhiko; Park, Su A.; Jee, Kyoung Soo; Kim, Jeong Koo.
In: Journal of Biomaterials Science, Polymer Edition, Vol. 14, No. 12, 01.12.2003, p. 1311-1321.Research output: Contribution to journal › Article
TY - JOUR
T1 - A study of compatibility between cells and biopolymeric surfaces through quantitative measurements of adhesive forces
AU - Kim, Young Jick
AU - Shin, Jung Woog
AU - Park, Ki Dong
AU - Lee, Jin Woo
AU - Yui, Nobuhiko
AU - Park, Su A.
AU - Jee, Kyoung Soo
AU - Kim, Jeong Koo
PY - 2003/12/1
Y1 - 2003/12/1
N2 - The mechanism of cell adhesion to biomaterials or components of the extracellular matrix is an important topic in the field of tissue engineering and related biotechnological processes. Many factors affect cell adhesion, and many biochemical and biological studies have attempted to identify their roles in the adhesion mechanism. Systematic studies of this nature require quantification of the adhesive force of a cell to identify the effect of a specific factor. However, most studies of cell adhesive force have used qualitative approaches. We propose a new technique for quantifying the force by which cells adhere to various biomaterial surfaces, which utilizes the relationship between the deflection of a cantilever beam and the required force. A micropipette was used as the cantilever beam. This technique was used to measure the attachment forces of chondrocytes seeded on three different biodegradable polymers commonly used in tissue engineering and medicine: poly E-carprolactone (PCL), poly(L-lactide) (PLLA) and poly(lactic-co-glycolic acid) (PGLA, L/G = 75: 25). The bond between the cells and the three polymers was evaluated using the quantified adhesive forces. The adhesive forces were also measured 8, 12, 24 h and 5 days after seeding the chondrocytes on the polymer surfaces. Results of statistical analysis showed that the cells attached to the PLLA had the strongest average attachment force for up to 24 h after seeding (P < 0.05).
AB - The mechanism of cell adhesion to biomaterials or components of the extracellular matrix is an important topic in the field of tissue engineering and related biotechnological processes. Many factors affect cell adhesion, and many biochemical and biological studies have attempted to identify their roles in the adhesion mechanism. Systematic studies of this nature require quantification of the adhesive force of a cell to identify the effect of a specific factor. However, most studies of cell adhesive force have used qualitative approaches. We propose a new technique for quantifying the force by which cells adhere to various biomaterial surfaces, which utilizes the relationship between the deflection of a cantilever beam and the required force. A micropipette was used as the cantilever beam. This technique was used to measure the attachment forces of chondrocytes seeded on three different biodegradable polymers commonly used in tissue engineering and medicine: poly E-carprolactone (PCL), poly(L-lactide) (PLLA) and poly(lactic-co-glycolic acid) (PGLA, L/G = 75: 25). The bond between the cells and the three polymers was evaluated using the quantified adhesive forces. The adhesive forces were also measured 8, 12, 24 h and 5 days after seeding the chondrocytes on the polymer surfaces. Results of statistical analysis showed that the cells attached to the PLLA had the strongest average attachment force for up to 24 h after seeding (P < 0.05).
UR - http://www.scopus.com/inward/record.url?scp=0347600678&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347600678&partnerID=8YFLogxK
U2 - 10.1163/156856203322599662
DO - 10.1163/156856203322599662
M3 - Article
C2 - 14870936
AN - SCOPUS:0347600678
VL - 14
SP - 1311
EP - 1321
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
SN - 0920-5063
IS - 12
ER -