A survey of protein tyrosine kinase mRNAs expressed in normal human melanocytes

S. T. Lee, K. M. Strunk, R. A. Spritz

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

We have used the reverse transcription-polymerase chain reaction to survey the repertoire of protein tyrosine kinases expressed in cultured normal human melanocytes, a differentiated cell type derived from the neural crest. We identified 25 different tyrosine kinase cDNAs among a total of 608 protein tyrosinase kinase-related cDNAs analyzed. Six encode receptor tyrosine kinases for known ligands, several of which have been implicated in controlling melanocyte proliferation in vitro. Two others encode apparent receptor tyrosine kinases for unknown ligands. Four encode known non-receptor tyrosine kinases and five encode previously identified anonymous protein tyrosine kinases. Of the eight other melanocyte-associated protein tyrosine kinases, most or all appear to be novel. These 25 protein tyrosine kinase genes exhibit distinct patterns of expression in cultured human melanocytes, human erythroleukemia cells, and a variety of normal human tissues. We mapped 16 of the corresponding protein tyrosine kinase genes to specific human chromosomes, identifying a total of 19 human genetic loci, some of which may constitute candidate genes for genetic disorders of mammalian development.

Original languageEnglish
Pages (from-to)3403-3410
Number of pages8
JournalOncogene
Volume8
Issue number12
Publication statusPublished - 1993 Jan 1

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Melanocytes
Protein-Tyrosine Kinases
Messenger RNA
Receptor Protein-Tyrosine Kinases
Complementary DNA
Genes
Ligands
Leukemia, Erythroblastic, Acute
Inborn Genetic Diseases
Genetic Loci
Monophenol Monooxygenase
Neural Crest
Medical Genetics
Human Chromosomes
Surveys and Questionnaires
Protein Kinases
Reverse Transcription
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Lee, S. T. ; Strunk, K. M. ; Spritz, R. A. / A survey of protein tyrosine kinase mRNAs expressed in normal human melanocytes. In: Oncogene. 1993 ; Vol. 8, No. 12. pp. 3403-3410.
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Lee, ST, Strunk, KM & Spritz, RA 1993, 'A survey of protein tyrosine kinase mRNAs expressed in normal human melanocytes', Oncogene, vol. 8, no. 12, pp. 3403-3410.

A survey of protein tyrosine kinase mRNAs expressed in normal human melanocytes. / Lee, S. T.; Strunk, K. M.; Spritz, R. A.

In: Oncogene, Vol. 8, No. 12, 01.01.1993, p. 3403-3410.

Research output: Contribution to journalArticle

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