Abstract
A novel fluorogenic probe for facile and efficient detection of a target protein that binds to a bioactive small molecule was developed. The probe was composed of a thiol-activated fluorogenic moiety bearing an alkyne tag and a protein binding ligand linked to a photoreactive protein labeling group. The new probe in conjunction with affinity chromatography and mass spectrometry was utilized to identify a target protein in cell lysates.
| Original language | English |
|---|---|
| Pages (from-to) | 6336-6339 |
| Number of pages | 4 |
| Journal | Chemical Communications |
| Volume | 58 |
| Issue number | 43 |
| DOIs | |
| Publication status | Published - 2022 May 6 |
Bibliographical note
Publisher Copyright:© 2022 The Royal Society of Chemistry
All Science Journal Classification (ASJC) codes
- Catalysis
- Electronic, Optical and Magnetic Materials
- Ceramics and Composites
- Chemistry(all)
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry