Spatiotemporal analysis of the inflammatory response has been limited by the difficulties of in vivo imaging and reconstitution of inflammation in vitro. Here, we present a novel method for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transmigration of neutrophils during the inflammatory process. This enabled us to concurrently characterize transendothelial migration behaviors of neutrophils under the influence of various inflammatory stimuli.
|Number of pages||5|
|Journal||Lab on a chip|
|Publication status||Published - 2012 Oct 21|
All Science Journal Classification (ASJC) codes
- Biomedical Engineering