A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils.

Sewoon Han, Ji Jing Yan, Yoojin Shin, Jessie J. Jeon, Jihee Won, Hyo Eun Jeong, Roger D. Kamm, Young-Joon Kim, Seok Chung

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Spatiotemporal analysis of the inflammatory response has been limited by the difficulties of in vivo imaging and reconstitution of inflammation in vitro. Here, we present a novel method for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transmigration of neutrophils during the inflammatory process. This enabled us to concurrently characterize transendothelial migration behaviors of neutrophils under the influence of various inflammatory stimuli.

Original languageEnglish
Pages (from-to)3861-3865
Number of pages5
JournalLab on a chip
Volume12
Issue number20
DOIs
Publication statusPublished - 2012 Jan 1

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Transendothelial and Transepithelial Migration
Lab-On-A-Chip Devices
Microfluidics
Assays
Neutrophils
Spatio-Temporal Analysis
Imaging techniques
Monitoring
Inflammation
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Han, S., Yan, J. J., Shin, Y., Jeon, J. J., Won, J., Jeong, H. E., ... Chung, S. (2012). A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils. Lab on a chip, 12(20), 3861-3865. https://doi.org/10.1039/c2lc40445a
Han, Sewoon ; Yan, Ji Jing ; Shin, Yoojin ; Jeon, Jessie J. ; Won, Jihee ; Jeong, Hyo Eun ; Kamm, Roger D. ; Kim, Young-Joon ; Chung, Seok. / A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils. In: Lab on a chip. 2012 ; Vol. 12, No. 20. pp. 3861-3865.
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Han, S, Yan, JJ, Shin, Y, Jeon, JJ, Won, J, Jeong, HE, Kamm, RD, Kim, Y-J & Chung, S 2012, 'A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils.', Lab on a chip, vol. 12, no. 20, pp. 3861-3865. https://doi.org/10.1039/c2lc40445a

A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils. / Han, Sewoon; Yan, Ji Jing; Shin, Yoojin; Jeon, Jessie J.; Won, Jihee; Jeong, Hyo Eun; Kamm, Roger D.; Kim, Young-Joon; Chung, Seok.

In: Lab on a chip, Vol. 12, No. 20, 01.01.2012, p. 3861-3865.

Research output: Contribution to journalArticle

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AU - Won, Jihee

AU - Jeong, Hyo Eun

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AU - Chung, Seok

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