The Antarctic flowering plant Deschampsia antarctica is highly sensitive to climate change and has shown rapid population increases during regional warming of the Antarctic Peninsula. Several studies have examined the physiological and biochemical changes related to environmental stress tolerance that allow D. antarctica to colonize harsh Antarctic environments; however, the molecular mechanisms of its responses to environmental changes remain poorly understood. To elucidate the survival strategies of D. antarctica in Antarctic environments, we investigated the functions of actin depolymerizing factor (ADF) in this species. We identified eight ADF genes in the transcriptome that were clustered into five subgroups by phylogenetic analysis. DaADF3, which belongs to a monocot-specific clade together with cold-responsive ADF in wheat, showed significant transcriptional induction in response to dehydration and cold, as well as under Antarctic field conditions. Multiple drought and low-temperature responsive elements were identified as possible binding sites of C-repeat-binding factors in the promoter region of DaADF3, indicating a close relationship between DaADF3 transcription control and abiotic stress responses. To investigate the functions of DaADF3 related to abiotic stresses in vivo, we generated transgenic rice plants overexpressing DaADF3. These transgenic plants showed greater tolerance to low-temperature stress than the wild-type in terms of survival rate, leaf chlorophyll content, and electrolyte leakage, accompanied by changes in actin filament organization in the root tips. Together, our results imply that DaADF3 played an important role in the enhancement of cold tolerance in transgenic rice plants and in the adaptation of D. antarctica to its extreme environment.
|Journal||Frontiers in Plant Science|
|Publication status||Published - 2021 Sept 28|
Bibliographical noteFunding Information:
This work was supported by “Post-Polar Genomics Project: Functional genomic study for securing of polar useful genes (PE21160),” funded by Korea Polar Research Institute (KOPRI), “Development of potential antibiotic compounds using polar organism resources (15250103, KOPRI Grant PM21030),” funded by the Ministry of Oceans and Fisheries, Korea, and the Basic Science Research Program, Project No. 2018R1A6A1A03025607 through the National Research Foundation (NRF), funded by the Ministry of Education, Korea.
© Copyright © 2021 Byun, Cui, Lee, Oh, Yoo, Lee, Kim and Lee.
All Science Journal Classification (ASJC) codes
- Plant Science